Lipoate-binding proteins and specific lipoate-protein ligases in microbial sulfur oxidation reveal an atpyical role for an old cofactor.

ELife
Xinyun CaoChristiane Dahl

Abstract

Many Bacteria and Archaea employ the heterodisulfide reductase (Hdr)-like sulfur oxidation pathway. The relevant genes are inevitably associated with genes encoding lipoate-binding proteins (LbpA). Here, deletion of the gene identified LbpA as an essential component of the Hdr-like sulfur-oxidizing system in the Alphaproteobacterium Hyphomicrobium denitrificans. Thus, a biological function was established for the universally conserved cofactor lipoate that is markedly different from its canonical roles in central metabolism. LbpAs likely function as sulfur-binding entities presenting substrate to different catalytic sites of the Hdr-like complex, similar to the substrate-channeling function of lipoate in carbon-metabolizing multienzyme complexes, for example pyruvate dehydrogenase. LbpAs serve a specific function in sulfur oxidation, cannot functionally replace the related GcvH protein in Bacillus subtilis and are not modified by the canonical E. coli and B. subtilis lipoyl attachment machineries. Instead, LplA-like lipoate-protein ligases encoded in or in immediate vicinity of hdr-lpbA gene clusters act specifically on these proteins.

References

May 13, 1999·International Journal of Systematic Bacteriology·I BryantsevaL Mityushina
Sep 27, 2000·Biochimica Et Biophysica Acta·M CianciR Berni
Apr 11, 2001·Proceedings of the National Academy of Sciences of the United States of America·N E Robinson, A B Robinson
Jan 1, 2004·Chemistry & Biology·Xin ZhaoJohn E Cronan
Jul 21, 2004·Journal of Computational Chemistry·Eric F PettersenThomas E Ferrin
Oct 14, 2005·Advances in Microbial Physiology·John E CronanYanfang Jiang
Oct 15, 1958·Proceedings of the National Academy of Sciences of the United States of America·J Spizizen
May 25, 2007·The Journal of Biological Chemistry·Nicholas R De Lay, John E Cronan
May 25, 2007·The Journal of Biological Chemistry·Véronique SauvéAndrew M Hemmings
Mar 28, 2008·Molecular Biology and Evolution·Si Quang Le, Olivier Gascuel
Apr 14, 2009·Methods in Enzymology·John E Cronan, Jacob Thomas
Jun 13, 2009·The Journal of Biological Chemistry·Quin H Christensen, John E Cronan
May 29, 2010·Microbiology and Molecular Biology Reviews : MMBR·Maroya D Spalding, Sean T Prigge
Oct 6, 2010·Plant Physiology·Robert E Blankenship
Oct 26, 2010·Journal of Biotechnology·Verena KallnikPaul Schweiger
Feb 23, 2011·Molecular Microbiology·Quin H ChristensenJohn E Cronan
Jun 21, 2011·Frontiers in Microbiology·Stefanie MangoldMark Dopson
Jan 29, 2013·Applied and Environmental Microbiology·Héctor OsorioDavid S Holmes
Mar 26, 2014·Biochimica Et Biophysica Acta·S S VenceslauI A C Pereira
Mar 24, 2016·Molecular Biology and Evolution·Sudhir KumarKoichiro Tamura
Apr 15, 2016·Microbiology and Molecular Biology Reviews : MMBR·John E Cronan

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Citations

Jan 25, 2019·Proceedings of the National Academy of Sciences of the United States of America·Simona G HuwilerMatthias Boll
Jun 13, 2019·Environmental Microbiology·Benjamin M ZeldesRobert M Kelly
Aug 11, 2020·Environmental Microbiology·James A CountsRobert M Kelly
Jan 29, 2019·Frontiers in Microbiology·Rui WangLin-Xu Chen
Jan 22, 2021·FEMS Microbiology Reviews·April M LewisRobert M Kelly
Mar 14, 2021·The Science of the Total Environment·Bo WuShanquan Wang
May 13, 2021·The Journal of Physical Chemistry. B·Daniel J Willard, Robert M Kelly

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Methods Mentioned

BETA
Assay
lipoylation
electrophoresis
PCR
ion exchange chromatography

Software Mentioned

UCSF Chimera
BioNJ
Model
MEGA7
BLASTP
SWISS

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