Lipocalin-type prostaglandin D synthase (beta-trace) is a newly recognized type of retinoid transporter.

The Journal of Biological Chemistry
T TanakaO Hayaishi

Abstract

Lipocalin-type prostaglandin D synthase is responsible for the biosynthesis of prostaglandin D2 in the central nervous system and the genital organs and is secreted into the cerebrospinal fluid and the seminal plasma as beta-trace. Here we analyzed retinoids binding of the enzyme by monitoring the fluorescence quenching of an intrinsic tryptophan residue, and appearance of circular dichroism around 330 nm, and a red shift of the UV absorption spectra of retinoids. We found that the enzyme binds all-trans- or 9-cis-retinoic acid and all-trans- or 13-cis-retinal, but not all-trans-retinol, with affinities (Kd of 70-80 nM) sufficient for function as a retinoid transporter. All-trans-retinoic acid inhibited the enzyme activity in a noncompetitive manner, suggesting that it binds to the same hydrophobic pocket as prostaglandin H2, the substrate for prostaglandin D synthase, but at a different site in this pocket. It is likely that this enzyme is a bifunctional protein that acts as both retinoid transporter and prostaglandin D2-producing enzyme.

References

May 17, 1976·European Journal of Biochemistry·U CoganM Shinitzky
Oct 1, 1992·Trends in Biochemical Sciences·M LeidP Chambon
Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·M IgarashiO Hayaishi
Oct 1, 1991·Trends in Biochemical Sciences·M C Peitsch, M S Boguski
May 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·A NagataO Hayaishi
Sep 1, 1989·Prostaglandins, Leukotrienes, and Essential Fatty Acids·S ItoO Hayaishi
Sep 1, 1987·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·S Pervaiz, K Brew
Sep 23, 1980·Biochimica Et Biophysica Acta·R D Fugate, P S Song
Feb 1, 1995·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M E Newcomer
Sep 15, 1994·Biochemical and Biophysical Research Communications·K WatanabeO Hayaishi
Dec 31, 1993·Brain Research·S DevR B Edwards
Oct 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·Y UradeO Hayaishi
Oct 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·M E NewcomerD E Ong
Aug 15, 1996·The Biochemical Journal·D R Flower
Oct 1, 1995·Journal of Lipid Mediators and Cell Signalling·Y UradeO Hayaishi
Sep 1, 1996·Journal of Lipid Mediators and Cell Signalling·Y UradeK Watanabe
May 1, 1961·Proceedings of the Society for Experimental Biology and Medicine·J CLAUSEN
Jan 1, 1992·Mediators of Inflammation·H TohT Tanabe

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Citations

Aug 10, 2000·Journal of Molecular Recognition : JMR·A Skerra
Sep 8, 2010·Modern Rheumatology·Hassan FahmiMohit Kapoor
Feb 16, 1999·Biochimica Et Biophysica Acta·Y Urade, O Hayaishi
May 22, 2002·Contraception·Maria Grazia LeoneLuciano Saso
Nov 4, 2000·Biochimica Et Biophysica Acta·G Lepperdinger
Nov 4, 2000·Biochimica Et Biophysica Acta·A Skerra
Nov 4, 2000·Biochimica Et Biophysica Acta·G GutiérrezD Sánchez
Nov 4, 2000·Biochimica Et Biophysica Acta·L KjeldsenN Borregaard
Sep 29, 2011·Chemical Reviews·William L SmithPer-Johan Jakobsson
Nov 28, 2001·European Journal of Biochemistry·I JerabekM Geiger
Nov 30, 2010·The Journal of Biological Chemistry·Karine MathurinJean-Luc Parent
Apr 21, 2010·Journal of Biochemistry·Kazuhisa TakedaShigeki Shibahara
Feb 10, 2000·Molecular Biology and Evolution·M D GanforninaD Sánchez
Nov 18, 2003·Reproductive Biology and Endocrinology : RB&E·Katherine G HamilSusan H Hall
Feb 23, 2008·Molecular Endocrinology·Jinhui ZhangXiaoli Chen
Nov 2, 2011·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Shigeru ShimamotoTadayasu Ohkubo
Mar 11, 2011·PloS One·Benjamin D WegerThomas Dickmeis
Jan 19, 2002·Clinical Chemistry and Laboratory Medicine : CCLM·B SuY Lu
Jun 30, 2012·Clinical Journal of the American Society of Nephrology : CJASN·Tariq ShafiJosef Coresh
Feb 7, 1998·Proceedings of the National Academy of Sciences of the United States of America·Y EguchiO Hayaishi
Apr 9, 2014·Nature Reviews. Urology·Andrei P DrabovichEleftherios P Diamandis
Aug 1, 2002·American Journal of Reproductive Immunology : AJRI·Shigeru SaitoToshihiko Michimata
Apr 30, 2013·Journal of Lipid Research·Sheila M O'Byrne, William S Blaner
Jan 14, 2004·The Journal of Obstetrics and Gynaecology Research·Yasuhiko ShikiYoshihiro Urade
Mar 23, 2007·Acta Neurologica Scandinavica·R AnckarsäterH Anckarsäter
Jul 14, 2012·Mediators of Inflammation·Myungsoo Joo, Ruxana T Sadikot

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