PMID: 9546674Apr 18, 1998Paper

Lipocalins of boar salivary glands binding odours and pheromones

European Journal of Biochemistry
S MarcheseP Pelosi

Abstract

Large amounts of an odorant-binding protein have been isolated from submaxillary glands of mature male pig. This polypeptide molecule is sex-specific, being absent in females. On electrophoretic gels under denaturing conditions it migrated as a broad band with an apparent molecular mass of around 20 kDa. Electrospray mass spectrometry revealed the presence of three main components, whose mass differences are not interpretable as result of any common post-translational modifications, indicating the presence of distinct polypeptide chains. N-terminal Edman degradation yielded a single sequence of 29 amino acids. It includes the lipocalin signature (-G-X-W-) and shows clear homology with a subclass of odorant-binding proteins present in mouse saliva, nasal mucus and urine. The purified protein still retained small ligands tightly bound; among them 5alpha-androst-16-en-3-one and 5alpha-androst-16-en-3alpha-ol, both known sex pheromones for the pig, were identified. The protein also binds 2-isobutyl-3-methoxypyrazine, a good ligand for most odorant-binding proteins, with a dissociation constant of 5 microM.

Citations

Nov 23, 2013·Applied Microbiology and Biotechnology·Paolo PelosiKrishna C Persaud
May 24, 2006·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Niels Borregaard, Jack B Cowland
May 23, 2009·Journal of Chemical Ecology·Chrystelle Le DanvicPatricia Nagnan-Le Meillour
Nov 4, 2000·Biochimica Et Biophysica Acta·M TegoniC Cambillau
Apr 20, 2013·Chemical Senses·Immacolata IovinellaPaolo Pelosi
Feb 4, 1999·Annals of the New York Academy of Sciences·P Pelosi
May 31, 2011·BMC Evolutionary Biology·Camille MeslinPhilippe Monget
Sep 26, 2008·PloS One·Darren W LoganLisa Stowers
Mar 19, 2014·Current Allergy and Asthma Reports·Wayne R Thomas
Nov 28, 2012·Veterinary Immunology and Immunopathology·A M GutiérrezI Miller
Mar 10, 2010·General and Comparative Endocrinology·Ponnirul PonmanickamGovindaraju Archunan
May 10, 2013·Journal of Neuropathology and Experimental Neurology·Paula Garcia-EsparciaIsidre Ferrer
May 27, 2008·Biochemical and Biophysical Research Communications·Sha LiPaolo Pelosi
Aug 12, 2008·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Eric B Keverne
Sep 18, 2004·Peptides·Loïc BriandJean-Claude Pernollet
Jul 23, 2013·Biochemical and Biophysical Research Communications·Liping BanPaolo Pelosi
Sep 16, 2014·Frontiers in Physiology·Paolo PelosiFrancesca R Dani
Jul 28, 2016·Frontiers in Aging Neuroscience·Isidro FerrerStefano Gustincich
Feb 24, 2018·Frontiers in Physiology·Jiao ZhuGuirong Wang
Oct 29, 2017·Proceedings of the National Academy of Sciences of the United States of America·Jiao ZhuPaolo Pelosi
Nov 3, 2017·Proceedings of the National Academy of Sciences of the United States of America·Walter S Leal
Jul 29, 2018·Applied Microbiology and Biotechnology·Paolo PelosiWolfgang Knoll
May 23, 2006·American Journal of Primatology·C S Evans
Oct 26, 2010·Rapid Communications in Mass Spectrometry : RCM·R RajkumarC C Liao
Aug 13, 2014·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Subramanian MuthukumarBalazs Gulyas
May 25, 2002·European Journal of Biochemistry·Silvia SpinelliChristian Cambillau
Jun 21, 2018·Scientific Reports·Subramanian MuthukumarGovindaraju Archunan
Jun 22, 2018·Journal of Animal Science and Biotechnology·Hakhyun KaJisoo Han
Nov 8, 2006·Biochemistry·Barbara D'InnocenzoPaolo Pelosi

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