Lipofuscin and N-retinylidene-N-retinylethanolamine (A2E) accumulate in retinal pigment epithelium in absence of light exposure: their origin is 11-cis-retinal.

The Journal of Biological Chemistry
Nicholas P BoyerYiannis Koutalos

Abstract

The age-dependent accumulation of lipofuscin in the retinal pigment epithelium (RPE) has been associated with the development of retinal diseases, particularly age-related macular degeneration and Stargardt disease. A major component of lipofuscin is the bis-retinoid N-retinylidene-N-retinylethanolamine (A2E). The current model for the formation of A2E requires photoactivation of rhodopsin and subsequent release of all-trans-retinal. To understand the role of light exposure in the accumulation of lipofuscin and A2E, we analyzed RPEs and isolated rod photoreceptors from mice of different ages and strains, reared either in darkness or cyclic light. Lipofuscin levels were determined by fluorescence imaging, whereas A2E levels were quantified by HPLC and UV-visible absorption spectroscopy. The identity of A2E was confirmed by tandem mass spectrometry. Lipofuscin and A2E levels in the RPE increased with age and more so in the Stargardt model Abca4(-/-) than in the wild type strains 129/sv and C57Bl/6. For each strain, the levels of lipofuscin precursor fluorophores in dark-adapted rods and the levels and rates of increase of RPE lipofuscin and A2E were not different between dark-reared and cyclic light-reared animals. Both 11-cis- a...Continue Reading

References

Apr 1, 1967·The Journal of Cell Biology·R W Young
May 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·B Kwok-Keung Fung, L Stryer
Jun 17, 1998·Free Radical Biology & Medicine·M RózanowskaT Sarna
Dec 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·C A ParishJ Sparrow
Jun 15, 2000·Proceedings of the National Academy of Sciences of the United States of America·N L MataG H Travis
Nov 9, 2000·Progress in Retinal and Eye Research·T Ebrey, Y Koutalos
Jan 5, 2002·The Journal of Biological Chemistry·Shimon Ben-ShabatJanet R Sparrow
Dec 24, 2003·Investigative Ophthalmology & Visual Science·Parisa ZamiriJ Wayne Streilein
Feb 7, 2004·Archives of Gerontology and Geriatrics·Martin L Katz, W Gerald Robison
May 19, 2004·The International Journal of Biochemistry & Cell Biology·Alexei Terman, Ulf T Brunk
May 3, 2005·Experimental Eye Research·Janet R Sparrow, Mike Boulton
Dec 1, 2007·Proceedings of the National Academy of Sciences of the United States of America·So R KimJanet R Sparrow
Apr 26, 2008·Molecular & Cellular Proteomics : MCP·Kwok-Peng NgJohn W Crabb
Feb 24, 2009·Biochimica Et Biophysica Acta·Robert S MoldayFaraz Quazi
Mar 7, 2009·Investigative Ophthalmology & Visual Science·Chunhe ChenYiannis Koutalos
Jun 26, 2009·Investigative Ophthalmology & Visual Science·Akiko MaedaKrzysztof Palczewski
Aug 12, 2009·Journal of Lipid Research·Janet R SparrowJilin Zhou
Oct 6, 2009·Vision Research·So Ra KimJanet R Sparrow
Apr 7, 2010·Proceedings of the National Academy of Sciences of the United States of America·Yalin WuJanet R Sparrow
Aug 11, 2010·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Chunhe Chen, Yiannis Koutalos
Oct 12, 2010·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Danielle B GutierrezZsolt Ablonczy
Mar 2, 2011·Investigative Ophthalmology & Visual Science·Angus C GreyZsolt Ablonczy
Mar 15, 2011·Investigative Ophthalmology & Visual Science·Lorie R BlakeleyYiannis Koutalos
Dec 21, 2011·The Journal of Biological Chemistry·Yu ChenKrzysztof Palczewski
Mar 16, 2012·Photochemistry and Photobiology·Nicholas P BoyerYiannis Koutalos

❮ Previous
Next ❯

Citations

Nov 26, 2013·Chemical Reviews·Rosana ÁlvarezÁngel R de Lera
Mar 20, 2013·International Journal of Inflammation·Ishaq A ViringipurampeerKevin Gregory-Evans
Jul 13, 2013·Investigative Ophthalmology & Visual Science·Zsolt AblonczyRosalie K Crouch
Jul 19, 2013·Investigative Ophthalmology & Visual Science·Jonathan P GreenbergFrançois C Delori
May 14, 2014·Journal of the American Society for Mass Spectrometry·David M G AndersonKevin L Schey
May 3, 2014·Quarterly Reviews of Biophysics·Peter M Jones, Anthony M George
Jan 1, 2013·Drug Discovery Today. Therapeutic Strategies·Konstantin Petrukhin
Sep 10, 2015·The Journal of Physiology·Alexander V KolesnikovVladimir J Kefalov
Nov 21, 2015·Proteomics. Clinical Applications·Hannah E BowreyZsolt Ablonczy
Apr 14, 2016·Proceedings of the National Academy of Sciences of the United States of America·Janet R Sparrow
Oct 30, 2012·Biochimica Et Biophysica Acta·Jonathan A ColemanRobert S Molday
Jul 4, 2015·Indian Journal of Ophthalmology·Andrea GabaiPaolo Lanzetta
Apr 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·Faraz Quazi, Robert S Molday
Jun 9, 2016·Proceedings of the National Academy of Sciences of the United States of America·Keiko UedaJanet R Sparrow
Jul 15, 2015·PloS One·Bill X WuCraig E Crosson
May 14, 2017·Photochemistry and Photobiology·Colleen SheridanYiannis Koutalos
Aug 1, 2015·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Patrick PallittoJulia Herrmann
Aug 7, 2016·Journal of Tissue Engineering·Aaron H Fronk, Elizabeth Vargis
Sep 2, 2015·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Leopold AdlerYiannis Koutalos
Jul 17, 2015·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Oliver P Ernst, Anant K Menon
Apr 28, 2018·Journal of Neuroscience Research·Maarjaliis PaavoJanet R Sparrow
Sep 6, 2018·Journal of Biomedical Optics·Lydia SauerMartin Hammer
Aug 22, 2018·Expert Opinion on Biological Therapy·Rehan M HussainByron L Lam
Sep 24, 2015·Retina·Dorothy A ThompsonMaite Tome Esteban
Jan 4, 2020·Cellular and Molecular Life Sciences : CMLS·Janusz Blasiak
May 23, 2020·Experimental Biology and Medicine·Tanu ParmarBeata Jastrzebska
Aug 20, 2020·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Chunhe ChenYiannis Koutalos
May 31, 2018·Investigative Ophthalmology & Visual Science·Fabian GarcesRobert S Molday
Nov 7, 2018·Proceedings of the National Academy of Sciences of the United States of America·Tamara L LenisRoxana A Radu
Jan 1, 2014·Journal of Clinical Medicine·Janet R Sparrow, Tobias Duncker
Sep 10, 2020·Journal of Clinical Medicine·Sander De BruyneJoris R Delanghe

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Related Papers

Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology
Danielle B GutierrezZsolt Ablonczy
Proceedings of the National Academy of Sciences of the United States of America
Nathan FishkinKoji Nakanishi
© 2021 Meta ULC. All rights reserved