Lipogenesis and lipoprotein lipase in human adipose tissue: reproducibility of measurements and relationships with fat cell size

Canadian Journal of Physiology and Pharmacology
R SavardC Bouchard

Abstract

Lipogenesis from glucose and lipoprotein lipase activity were investigated in humans. The reliability of measurements was quantified and correlations with fat cell weight were assessed. Twenty-four subjects (7 women, 17 men) were studied twice within a 2-week period, along with 17 additional male subjects who were studied once and used only in the correlation analyses. All subjects were not regularly involved in an exercise-training program and were between 18 and 30 years of age. Following an overnight fast, adipose tissue specimens were obtained by suprailiac biopsy and fat cells were collagenase isolated. Mean fat cell weight was obtained from 400 to 500 cell diameter determinations per subject. Basal and insulin-stimulated fat cell lipogenesis from glucose were determined using D-[U-14C]glucose and were reported in nanomoles of glucose per hour per 10(6) cells. Adipose tissue heparin-releasable lipoprotein lipase activity was also determined and expressed in micromoles of free fatty acids per hour per gram of tissue and per 10(6) cells. Fat cell weight, basal and insulin-stimulated lipogenesis and lipoprotein lipase activity per gram showed high reliability of measurement, interclass and intraclass coefficients being 0.83 a...Continue Reading

Citations

Jan 1, 1987·European Journal of Applied Physiology and Occupational Physiology·E T PoehlmanC Bouchard
Aug 1, 1987·Baillière's Clinical Endocrinology and Metabolism·M R Taskinen, T Kuusi
Feb 1, 1988·Archives of Disease in Childhood·L M RovamoK O Raivio
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Dec 1, 1996·Endocrinology and Metabolism Clinics of North America·T G Ramsay
Mar 21, 2003·Metabolism: Clinical and Experimental·P MauriègeY Deshaies
Jul 1, 1989·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·J P DesprésC Bouchard
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Dec 1, 1988·European Journal of Clinical Investigation·O Andersen, C Kühl
May 1, 1987·Metabolism: Clinical and Experimental·R SavardC Bouchard

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