Lipopeptide epitopes extended by an Nepsilon-palmitoyl-lysine moiety increase uptake and maturation of dendritic cells through a Toll-like receptor-2 pathway and trigger a Th1-dependent protective immunity

European Journal of Immunology
Xiaoming ZhuLbachir BenMohamed

Abstract

Lipopeptides, a form of peptide immunogens, are currently under intense investigation as human vaccines for many infectious pathogens and cancers. However, the cellular and molecular mechanisms of lipopeptide immunogenicity are only partially understood. We have investigated the influence of the lipid content on the immunogenicity of lipopeptides using the herpes simplex virus type 1 (HSV-1) gD(1-23) peptide as a model antigen. Totally synthetic lipopeptides were constructed by covalent attachment to the peptide backbone of either Nepsilon-palmitoyl-lysine (palmitoyl-lipidated peptide, palmitoyl-LP) or cholesterol-lysine (cholesterol-lipidated peptide, cholesterol-LP). Immunization of mice with the palmitoyl-LP, but not with its cholesterol-LP analog, induced a strong T cell-dependent protective immunity against lethal HSV-1 infection. Analysis of cytokine profiles and IgG2a/IgG1 ratios revealed that a dominant Th1-type immune response was stimulated by the palmitoyl-LP, as opposed to a Th2 response generated by its cholesterol-LP analog. The palmitoyl-LP was efficiently taken up in vitro by immature dendritic cells (DC) in a time- and dose-dependent manner, and induced phenotypic maturation and production of pro-inflammatory c...Continue Reading

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Citations

Oct 30, 2009·Archivum Immunologiae Et Therapiae Experimentalis·Aziz Alami ChentoufiLbachir BenMohamed
Jun 8, 2013·Amino Acids·Alessandro GoriGiorgio Colombo
Mar 12, 2013·European Journal of Pharmacology·Tabasum SidiqRakesh Kamal Johri
Feb 28, 2009·Biochemistry·Dimitris MissirlisMatthew Tirrell
Jun 14, 2013·AIDS·Laura RichertUNKNOWN ANRS Vaccine Network/Vaccine Research Institute
Sep 19, 2008·Journal of Virology·Aziz Alami ChentoufiLbachir BenMohamed
May 2, 2012·Clinical & Developmental Immunology·Aziz Alami ChentoufiLbachir Benmohamed
Dec 22, 2009·Future Microbiology·Gargi DasguptaLbachir BenMohamed
Jul 25, 2014·Journal of Immunology Research·Afonso P Basto, Alexandre Leitão
Jan 15, 2013·Drug Delivery and Translational Research·Mehfuz ZamanIstvan Toth
Jan 16, 2013·Clinical & Developmental Immunology·Aziz Alami Chentoufi, Lbachir Benmohamed
Feb 22, 2012·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Souichi OomizuMitsuomi Hirashima
Aug 19, 2007·Vaccine·Michael KargakisEugenia Panou-Pomonis
Mar 10, 2017·Biomaterials Science·Carlo PifferiOlivier Renaudet
Feb 3, 2015·Current Protocols in Microbiology·Peter Michael Moyle
Nov 30, 2006·Journal of Peptide Science : an Official Publication of the European Peptide Society·Alfonso BavosoAlfonso Tramontano
Jul 25, 2009·Expert Review of Vaccines·Gargi DasguptaLbachir BenMohamed
Aug 1, 2008·Clinical and Vaccine Immunology : CVI·Xiuli ZhangLbachir BenMohamed
Feb 14, 2007·Reviews in Medical Virology·Adi ReskeDavid R Katz
Feb 4, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Aziz A ChentoufiLbachir BenMohamed
Sep 29, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Xiuli ZhangLbachir Benmohamed
Jan 15, 2013·ChemMedChem·Peter Michael Moyle, Istvan Toth
Sep 8, 2017·Expert Review of Vaccines·Mathieu SurenaudJean-Daniel Lelièvre
Dec 22, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Aziz Alami ChentoufiLbachir BenMohamed

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