Lipopeptide immunization without adjuvant induces potent and long-lasting B, T helper, and cytotoxic T lymphocyte responses against a malaria liver stage antigen in mice and chimpanzees

European Journal of Immunology
L BenMohamedP Druilhe

Abstract

We have employed a 26-amino-acid synthetic peptide based on Plasmodium falciparum liver stage antigen-3 to evaluate improvements in immunogenicity mediated by the inclusion of a simple lipid-conjugated amino acid during peptide synthesis. Comparative immunization by the peptide in Freund's adjuvant or by the lipopeptide in saline shows that the addition of a palmitoyl chain can dramatically increase T helper (Th) cell responses in a wide range of major histocompatibility complex (MHC) class II haplotypes, to the extent that responses were induced in mice otherwise unable to respond to the non-modified peptide injected with Freund's adjuvant, and that the increased immunogenicity of the lipopeptide led to high and longer lasting antibody production (studied up to 8 months). B and T cell responses induced by the lipopeptide were reactive with native parasite protein epitopes, and a lipopeptide longer than ten amino acids was endogenously processed to associate with MHC class I and elicit cytotoxic T lymphocyte (CTL) responses. Finally, the lipopeptide was safe and highly immunogenic in chimpanzees, whose immune system is very similar to that of humans. Our results suggest that relatively large synthetic peptides, carefully chosen...Continue Reading

References

Nov 1, 1991·European Journal of Immunology·H SchildH G Rammensee
Dec 22, 1990·Proceedings. Biological Sciences·K J RobsonT E Wellems
Mar 1, 1990·Immunology Today·G Gregoriadis
Apr 2, 1987·Journal of Immunological Methods·K LövgrenB Morein
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J P Tam
Jan 1, 1972·Advances in Experimental Medicine and Biology·R Arnon
May 1, 1984·The American Journal of Tropical Medicine and Hygiene·P DruilheM Gentilini
Apr 25, 1995·Proceedings of the National Academy of Sciences of the United States of America·L F Scheller, A F Azad
Apr 1, 1994·Molecular and Biochemical Parasitology·D A FidockA Asavanich
Jan 1, 1996·The American Journal of Tropical Medicine and Hygiene·P DaubersiesP Druilhe
Dec 1, 1993·Current Biology : CB·T ElliottA McMichael
Feb 1, 1989·Parasitology Today·O B Akogun
Jan 1, 1991·Parasitology Today·A Suhrbier

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Citations

Oct 30, 2009·Archivum Immunologiae Et Therapiae Experimentalis·Aziz Alami ChentoufiLbachir BenMohamed
Jun 28, 2008·Cancer Immunology, Immunotherapy : CII·Ilham BettahiLbachir BenMohamed
May 29, 2000·Journal of Controlled Release : Official Journal of the Controlled Release Society·W QuintilioM H Bueno da Costa
Jun 18, 2002·Bioorganic & Medicinal Chemistry Letters·Elisa PeroniAnna M Papini
Jan 22, 2003·Immunology and Cell Biology·Brendon Y ChuaDavid C Jackson
Jun 27, 2001·AIDS·G PialouxUNKNOWN ANRS VAC 04 Study Team
Jan 14, 2009·Infection and Immunity·Aissatou Toure-BaldePierre Druilhe
Jul 20, 2002·The Lancet Infectious Diseases·Lbachir BenMohamedAnthony B Nesburn
Jul 15, 2015·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Renuka Ramanathan, Kim Woodrow

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