Lipopeptide stimulation of MHC class I-restricted memory cytotoxic T lymphocytes from equine infectious anemia virus-infected horses

Vaccine
Sherritta L Ridgely, T C McGuire

Abstract

The immunogenicity of equine infectious anemia virus (EIAV) Gag and Env equine leukocyte alloantigen (ELA)-A5.1, -A9, and -A1 restricted cytotoxic T lymphocyte (CTL) epitopes synthesized on multiple antigenic peptide (MAP) system coupled to tripalmitoyl-S-glycerylcysteine (P3C) was evaluated in vitro. P3C-MAP-peptide-stimulated peripheral blood mononuclear cells (PBMCs) from horses, chronically infected with EIAV, had memory CTL (CTLm) similar to that of PBMCs stimulated with either the minimal CTL epitopes, longer peptides containing the CTL epitopes, or EIAV. The stimulated CTL lysed EIAV-infected target cells and the percent specific lysis was dependent on the dose of P3C-MAP-peptide used to stimulate PBMCs in vitro and was peptide specific and ELA-A restricted. The results suggest that these P3C-MAP-peptides can be used as immunogens to stimulate primary immune responses in vivo.

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Citations

Feb 15, 2003·Veterinary Immunology and Immunopathology·Eliane MartiD Paul Lunn
Jun 30, 2005·Animal Health Research Reviews·Travis C McGuireRobert H Mealey
Jul 25, 2014·Journal of Immunology Research·Afonso P Basto, Alexandre Leitão
Oct 1, 2005·Journal of Peptide Science : an Official Publication of the European Peptide Society·Petr NiederhafnerJan Jezek
Sep 19, 2003·The Journal of General Virology·Gisela SobollD Paul Lunn

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