PMID: 7581337Sep 1, 1995Paper

Lipophosphoglycan is present in distinctly different form in different Entamoeba histolytica strains and absent in Entamoeba moshkovskii and Entamoeba invadens

The Journal of Eukaryotic Microbiology
G SrivastavaA Bhattacharya

Abstract

Lipophosphoglycan has recently been demonstrated on the cell surface of Entamoeba histolytica strain HM-1:IMSS. A monoclonal antibody against this molecule had failed to react with some other strains of E. histolytica, including the strain Rahman. To determine if a structurally distinct lipophosphoglycan existed in Rahman, [3H]galactose-labeled glycoconjugates were electrophoresed through sodium dodecyl sulfate polyacrylamide gel electrophoresis. The electrophoretic pattern in Rahman was very different compared to that obtained with strains HM-1:IMSS and 200:NIH. A number of experiments including sensitivity to mild acid, nitrous acid and phosphoinositol-specific phospholipase C suggest that the Rahman glycoconjugate is indeed a lipophosphoglycan-like molecule but distinctly different from that of HM-1:IMSS. Mild acid-treated glycoconjugates from Rahman and HM-1:IMSS revealed the presence of neutral trisaccharides and monosaccharides in Rahman but not in HM-1:IMSS. Human immune sera from amoebiasis patients and a polyclonal antibody against HM-1:IMSS lipophosphoglycan both recognized Rahman glycoconjugate. Thus, while lipophosphoglycan molecules from the two strains share common epitopes, they are clearly distinct from each oth...Continue Reading

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Citations

Jun 14, 2002·International Journal for Parasitology·Siddhartha DasKrishna Reddy
Jan 30, 2003·Molecular and Biochemical Parasitology·Ranjana AryaAlok Bhattacharya
Feb 19, 2011·Journal of Parasitology Research·Adam Sateriale, Christopher D Huston
Jul 30, 2014·Gut and Liver·María S FloresLuis Galán
Apr 9, 2005·Parasite Immunology·Rafael Campos-Rodríguezp, Adriana Jarillo-Luna
Mar 15, 2000·Journal of Molecular Biology·S Moody-HauptM J McConville

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