Lipopolysaccharide activates endothelial nitric oxide synthase through protein tyrosine kinase

Biochemical and Biophysical Research Communications
K T HuangJ C Liao

Abstract

Vascular endothelial cell injury or activation by lipopolysaccharide (LPS) plays an important role in the pathogenesis of endotoxin shock. However, the effect of LPS on NO production from vascular endothelial cells (ECs) is incompletely understood. In this study, bovine coronary venular ECs were treated with LPS and the release of NO and expression of the endothelial NO synthase (ecNOS) were examined. We found that the ecNOS activity is transiently enhanced by LPS within the time scale of about 10 h due to the interplay between two LPS-induced mechanisms. Within the first 10 h of LPS treatment, the specific activity of ecNOS is increased by a post-translational modification mediated through a protein tyrosine kinase cascade. After about 10 h of treatment, however, LPS destabilizes the transcript of ecNOS and thus decreased the expression level and total activity.

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Citations

Jun 3, 1999·American Journal of Respiratory and Critical Care Medicine·A S ComtoisS N Hussain
May 22, 2010·Journal of Clinical Biochemistry and Nutrition·Shuzo OhataTatsuya Matsura
Mar 14, 2013·World Journal of Gastroenterology : WJG·Dong Chuan ZuoJae Yeoul Jun
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