Lipopolysaccharide acutely inhibits proliferation of neural precursor cells in the dentate gyrus in adult rats

Brain Research
Hitomi Fujioka, Tatsuo Akema

Abstract

Lipopolysaccharide (LPS), a bacterial endotoxin released during infection, is known to suppress neurogenesis in the dentate gyrus (DG) in mature rats. The present study aimed to elucidate acute effect of LPS, as well as possible mechanisms involved in the effect, on the neurogenesis in the DG of adult rats. In the first experiment, proliferating cells in the DG were labeled with bromodeoxyuridine (BrdU). Double-labeled immunohistochemistry performed 28 days after the BrdU incorporation revealed co-expression of NeuN, a marker of mature neurons, in most of the BrdU-positive cells in the DG. The rat was injected intraperitoneally with LPS or saline at various intervals after the BrdU incorporation, and BrdU-positive cells were examined 24h thereafter. The endotoxin reduced the number of BrdU-positive cells that were labeled 24h before, but not 7 or 28 days before sacrifice, suggesting rapid LPS actions on precursor cells during proliferation, but not after mitosis. In the second experiment, cells in the DG positively stained with BrdU or serine10 phosphorylated histone H3 (pHH3) were examined 5h after the injection of LPS or saline. BrdU was incorporated 2h before sacrifice. In these rats, LPS reduced the number of BrdU- or pHH3-...Continue Reading

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