Lipopolysaccharide and lipoteichoic acid enhance serum amyloid A3 mRNA expression in murine alveolar epithelial cells

The Journal of Veterinary Medical Science
Ami IwataYasuo Inoshima

Abstract

Serum amyloid A (SAA) is an acute-phase protein indicative of inflammation. In murine colonic epithelial cells, lipopolysaccharide (LPS), a gram-negative bacterial antigen, strongly enhanced mRNA expression of SAA3, but not SAA1 or SAA2, suggesting that SAA3 might respond to bacterial infection in other epithelia. We examined SAA1/2 and SAA3 mRNA expression in murine alveolar epithelial cells exposed to LPS or the gram-positive bacterial antigen, lipoteichoic acid (LTA), using real-time PCR. LPS enhanced SAA3 mRNA expression at lower concentrations than did LTA, whereas SAA1/2 mRNA expression was not enhanced by either LPS or LTA. These results suggest that SAA3 expression is enhanced in lung epithelium upon bacterial infection as part of innate immunity, with higher sensitivity to LPS than to LTA.

References

Dec 1, 1986·The Journal of Experimental Medicine·R L Meek, E P Benditt
May 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·D N CarneyJ D Minna
Dec 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·K A WikenheiserJ A Whitsett
Oct 26, 2005·Nature Medicine·Dianhua JiangPaul W Noble
Mar 1, 2006·BMC Bioinformatics·Joshua S YuanC Neal Stewart
Sep 23, 2008·Proceedings of the National Academy of Sciences of the United States of America·Malin E V JohanssonGunnar C Hansson
Jul 14, 2010·The Veterinary Journal·P D Eckersall, R Bell
Dec 3, 2010·The New England Journal of Medicine·John V Fahy, Burton F Dickey
Apr 4, 2014·The Journal of Veterinary Medical Science·Hiroaki ShigemuraYasuo Inoshima

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Citations

Aug 28, 2020·Experimental Lung Research·Haruka KawasakiYasuo Inoshima
Jun 3, 2021·Animals : an Open Access Journal From MDPI·Sato KamiyaYasuo Inoshima

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Methods Mentioned

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