Lipopolysaccharide and lipotheicoic acid differentially modulate epididymal cytokine and chemokine profiles and sperm parameters in experimental acute epididymitis

Scientific Reports
Erick J R SilvaMaria Christina W Avellar

Abstract

Bacterial infections are the most prevalent etiological factors of epididymitis, a commonly diagnosed inflammatory disease in the investigation of male infertility factors. The influence of early pathogenic mechanisms at play during bacterial epididymitis on reproductive outcomes is little understood. We report here that experimental epididymitis induced in rats by Gram-negative (LPS) and Gram-positive (LTA) bacterial products resulted in differential patterns of acute inflammation in the cauda epididymis. LPS elicited a strong inflammatory reaction, as reflected by upregulation of levels of mRNA for seven inflammatory mediators (Il1b, Tnf, Il6, Ifng, Il10, Nos2 and Nfkbia), and tissue concentration of six cytokines/chemokines (IL1A, IL1B, IL6, IL10, CXCL2 and CCL2) within the first 24 h post-treatment. Conversely, LTA induced downregulation of one (Nfkbia) and upregulation of six (Il1b, Il6, Nos2, Il4 Il10 and Ptgs1) inflammatory gene transcripts, whereas increased the tissue concentration of three cytokines/chemokines (IL10, CXCL2 and CCL2). The stronger acute inflammatory response induced by LPS correlated with a reduction of epididymal sperm count and transit time that occurred at 1, 7, and 15 days post-treatment. Our study...Continue Reading

References

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Citations

Feb 6, 2020·Molecular Human Reproduction·Britta KleinAndreas Meinhardt
Jun 21, 2019·Biopreservation and Biobanking·Razia IshaqShamim Akhter
Oct 23, 2019·Journal of Cell Science·Maria A BattistoneSylvie Breton
May 10, 2020·Cell and Tissue Research·Rukmali WijayarathnaMark P Hedger
Dec 1, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Michal DurackaEva Tvrda
Oct 10, 2020·Biology of Reproduction·Alexandre D AndradeErick J R Silva
Dec 18, 2020·Frontiers in Immunology·Christiane PleugerAndreas Meinhardt

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Methods Mentioned

BETA
PCR
electrophoresis
protein assay
ELISA

Software Mentioned

Image
GraphPad
Analyst
JColorGrid
7500 Biosystems
GraphPad Prism
Pro Express

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