Lipopolysaccharide binding protein from normal human plasma purified with high efficiency

Protein Expression and Purification
A NanboS Nagasawa

Abstract

Lipopolysaccharide (LPS)-binding protein (LBP) is an acute-phase plasma glycoprotein of 60 kDa which functions as an opsonin on activation of macrophages by bacterial LPS. The importance of LBP on the host defense against infection has been demonstrated. Human LBP has been purified from ascitic fluid or acute-phase serum. However, clinical samples often are not available, so we developed a method to purify LBP from normal plasma. The purification was accomplished by barium citrate precipitation, followed by three-step ion-exchange chromatography. The final step was Mono S cation-exchange chromatography, following Bio-Rex 70 and Mono Q chromatography, and it enabled the specific activity to increase overall 48,000-fold. This seems to be the first report of the use of Mono S column to purify LBP. The LBP obtained using these steps proved to be homogeneous by SDS-PAGE, Western blotting, and N-terminal sequence and amino acid composition analyses. The purification method established here will compensate when normal plasma with a low LBP content is available as the starting source.

References

Sep 21, 1990·Science·R R SchumannR J Ulevitch
Sep 1, 1986·The Journal of Experimental Medicine·P S TobiasR J Ulevitch
May 11, 1988·Nucleic Acids Research·E Ferrero, S M Goyert
Apr 1, 1973·Virology·F L Graham, A J van der Eb
Jan 1, 1995·Annual Review of Immunology·R J Ulevitch, P S Tobias
Feb 1, 1993·Infection and Immunity·P GallayD Heumann
Dec 1, 1994·Journal of Leukocyte Biology·L S KhemlaniP N Bochsler
Jun 1, 1996·Veterinary Immunology and Immunopathology·P N BochslerR C Carroll

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.