Lipopolysaccharide-binding protein (LBP) can reverse the amyloid state of fibrin seen or induced in Parkinson's disease

PloS One
Etheresia PretoriusDouglas B Kell

Abstract

The thrombin-induced polymerisation of fibrinogen to form fibrin is well established as a late stage of blood clotting. It is known that Parkinson's Disease (PD) is accompanied by dysregulation in blood clotting, but it is less widely known as a coagulopathy. In recent work, we showed that the presence of tiny amounts of bacterial lipopolysaccharide (LPS) in healthy individuals could cause clots to adopt an amyloid form, and this could be observed via scanning electron microscopy (SEM) or via the fluorescence of thioflavin-T. This could be prevented by the prior addition of lipopolysaccharide-binding protein (LBP). We had also observed by SEM this unusual clotting in the blood of patients with Parkinson's Disease. We hypothesised, and here show, that this too can be prevented by LBP in the context of PD. This adds further evidence implicating inflammatory microbial cell wall products as an accompaniment to the disease, and may be part of its aetiology. This may lead to novel treatment strategies in PD designed to target microbes and their products.

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Citations

Mar 1, 2019·Scientific Reports·Martin J PageEtheresia Pretorius
Oct 27, 2018·International Journal of Molecular Sciences·Anita Mikołajczyk, Dagmara Złotkowska
Nov 23, 2019·Current Topics in Medicinal Chemistry·Maria J E Visser, Etheresia Pretorius
Jun 22, 2020·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Ingar OlsenEtheresia Pretorius
Aug 23, 2020·Life·Maria S RubelAleksandr A Rubel
Sep 12, 2019·Frontiers in Aging Neuroscience·Büin AdamsEtheresia Pretorius
Mar 27, 2018·Biological Reviews of the Cambridge Philosophical Society·Douglas B Kell, Etheresia Pretorius
May 1, 2021·Biomolecules·Nathan A ShlobinChaim G Pick
Oct 9, 2021·Seminars in Thrombosis and Hemostasis·Martin J Page, Etheresia Pretorius

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Methods Mentioned

BETA
X-ray
scanning electron microscopy
confocal microscopy
scanning
electron microscopy
super-resolution microscopy

Software Mentioned

Airyscan
GraphPad
ImageJ
Excel

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