PMID: 8943264Dec 13, 1996Paper

Lipopolysaccharide biosynthesis in Rhizobium leguminosarum. Novel enzymes that process precursors containing 3-deoxy-D-manno-octulosonic acid.

The Journal of Biological Chemistry
K A BrozekChristian R H Raetz

Abstract

The lipopolysaccharide of Rhizobium leguminosarum differs from that of other Gram-negative organisms. R. leguminosarum lipid A lacks phosphate groups, but it contains a galacturonic acid residue at the 4'-position and an aminogluconate moiety in place of the usual glucosamine 1-phosphate unit. R. leguminosarum lipid A is esterified with a peculiar long chain fatty acid, 27-hydroxyoctacosanoate, not found in enteric Gram-negative bacteria, and the inner core of R. leguminosarum contains mannose and galactose in place of heptose. Despite these differences, the biosynthesis of R. leguminosarum lipid A is initiated by the same seven enzyme pathway as in Escherichia coli (Raetz, C. R. H. (1993) J. Bacteriol. 175, 5745-5753) to form the phosphorylated precursor, (Kdo)2-lipid IVA, which is then processed differently. We now describe several novel Rhizobium-specific enzymes that recognize and modify (Kdo)2-lipid IVA. The 1- and 4'-phosphatases were detected using (Kdo)2-[1-32P]-lipid IVA and (Kdo)2-[4'-32P]-lipid IVA, respectively, as shown by release of 32Pi. In the presence of GDP-mannose and/or UDP-galactose, membranes of R. leguminosarum first transferred mannose and then galactose to (Kdo)2-[4'-32P]-lipid IVA. In addition, at leas...Continue Reading

References

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Feb 1, 1994·Current Opinion in Immunology·R J Ulevitch, P S Tobias
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Citations

Jul 25, 2012·Proceedings of the National Academy of Sciences of the United States of America·Ryan P EmptageChristian R H Raetz
May 23, 2002·The Journal of Biological Chemistry·Shib Sankar BasuChristian R H Raetz
Jan 18, 2003·The Journal of Biological Chemistry·Nanette L S Que-GewirthChristian R H Raetz
Jan 18, 2003·The Journal of Biological Chemistry·Nanette L S Que-GewirthChristian R H Raetz
Jul 19, 2003·The Journal of Biological Chemistry·Mark J KarbarzChristian R H Raetz
Jul 5, 2005·The Journal of Biological Chemistry·Middleton Boon HinckleyChristian R H Raetz
Feb 25, 2006·The Journal of Biological Chemistry·Suparna Kanjilal-KolarChristian R H Raetz
Feb 25, 2006·The Journal of Biological Chemistry·Suparna Kanjilal-Kolar, Christian R H Raetz
Feb 10, 2006·The Journal of Biological Chemistry·Xiaoyuan WangChristian R H Raetz
Nov 6, 2008·The Journal of Biological Chemistry·Mark J KarbarzChristian R H Raetz
Mar 20, 2004·European Journal of Biochemistry·Adam Choma, Pawel Sowinski
Jun 5, 2002·Annual Review of Biochemistry·Christian R H Raetz, Chris Whitfield
Mar 17, 2007·Annual Review of Biochemistry·Christian R H RaetzRussell E Bishop
Mar 12, 2013·Annual Review of Biochemistry·William DowhanPaul Modrich
Apr 16, 2013·Biochimica Et Biophysica Acta·Christian SohlenkampBrian O Ingram
Apr 29, 2015·PloS One·Akintunde EmiolaSteven S Andrews
Jul 11, 2007·Journal of Endotoxin Research·Mari-Anne NewmanMichelangelo Parrilli
Dec 13, 1996·The Journal of Biological Chemistry·K A BrozekC R Raetz

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