Abstract
Microtubule disassembly is commonly believed to be a process of endwise tubulin dimer release. The present study demonstrates by video interference contrast microscopy that Escherichia coli lipopolysaccharide (LPS) caused microtubule disassembly in vitro by both endwise shortening and fragmentation. In contrast, the microtubules were only shortened from their ends in the presence of DNA, used as another example of a macromolecular microtubule effector. LPS-caused microtubule fragmentation was confirmed by transmission electron microscopy. Because of its ability to induce both fragmentation and endwise shortening, LPS, which is involved in sepsis pathogenesis, has to be regarded as a highly active microtubule-destabilizing agent.
References
Oct 16, 1978·Biochemical and Biophysical Research Communications·W VaterE Unger
Aug 1, 1992·The Journal of Cell Biology·S Kamimura, E Mandelkow
May 26, 1992·Biochemistry·L Eichinger, M Schleicher
Aug 1, 1992·The Journal of Cell Biology·C Febvre-Chevalier, J Febvre
Feb 22, 1991·Cell·R D Vale
Jul 6, 1987·Biochimica Et Biophysica Acta·K J BöhmE Unger
Mar 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·M L ShelanskiC R Cantor
Mar 1, 1980·European Journal of Biochemistry·V G CorcesG Wiche
Jul 30, 1984·Biochimica Et Biophysica Acta·K J BöhmE Unger
Jun 1, 1995·Microscopy Research and Technique·C Risco, P Pinto da Silva
Oct 14, 1994·Science·N ShiinaE Nishida
Nov 5, 1993·Cell·F J McNally, R D Vale
Apr 7, 1993·Molecular and Cellular Biochemistry·C RiscoJ L Carrascosa
Jan 1, 1997·Cell Motility and the Cytoskeleton·K J BöhmE Unger
Citations
Jan 7, 2000·Cell Biology International·K J BöhmE Unger
Nov 18, 2000·Biochimica Et Biophysica Acta·R DziarskiD Gupta
Sep 15, 2004·Mutation Research·Thomas StoiberEberhard Unger
Jan 7, 2015·Pharmacological Reports : PR·Manu Lopus, Pradeep Kumar Naik
Aug 9, 2017·Neurochemical Research·WenChong SunZuodi Liang
Nov 15, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·N IsowaM Liu