PMID: 8943265Dec 13, 1996Paper

Lipopolysaccharide core glycosylation in Rhizobium leguminosarum. An unusual mannosyl transferase resembling the heptosyl transferase I of Escherichia coli.

The Journal of Biological Chemistry
J L KadrmasChristian R H Raetz

Abstract

The lipopolysaccharide structure of the nitrogen-fixing bacterium Rhizobium leguminosarum differs from that of Escherichia coli in several ways, one of which is the sugar composition of the core. The E. coli inner core consists of 3-deoxy-D-manno-octulosonic acid (Kdo) and L-glycero-D-manno-heptose (heptose), while the inner core of R. leguminosarum contains 2-keto-3-deoxy-D-manno-octulosonic acid (Kdo), mannose, galactose, and galacturonic acid. The two Kdo residues and their linkages appear to be identical in both species. The linkages of heptose in E. coli and of mannose in R. leguminosarum to Kdo are both alpha1-5. We now characterize a membrane-associated glycosyl transferase in R. leguminosarum extracts that incorporates mannose into nascent lipopolysaccharide, using Kdo2-lipid IVA as the acceptor and GDP-mannose (or synthetic ADP-mannose) as the donor. The mannosyl transferase is associated with the inner membrane. The apparent Km values for GDP-mannose and Kdo2-lipid IVA are 4.3 microM and 7.1 microM, respectively, in the presence of excess co-substrate. Extracts of E. coli do not catalyze GDP-mannose-dependent glycosylation of Kdo2-lipid IVA, but they are active when ADP-mannose is substituted for GDP-mannose. Given th...Continue Reading

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Citations

Jun 12, 1999·The Plant Journal : for Cell and Molecular Biology·M C SuhJ B Ohlrogge
Mar 27, 2004·Proceedings of the National Academy of Sciences of the United States of America·Gail P FergusonGraham C Walker
Jul 25, 2012·Proceedings of the National Academy of Sciences of the United States of America·Ryan P EmptageChristian R H Raetz
May 23, 2002·The Journal of Biological Chemistry·Shib Sankar BasuChristian R H Raetz
Jan 18, 2003·The Journal of Biological Chemistry·Nanette L S Que-GewirthChristian R H Raetz
Jan 18, 2003·The Journal of Biological Chemistry·Nanette L S Que-GewirthChristian R H Raetz
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Jul 5, 2005·The Journal of Biological Chemistry·Middleton Boon HinckleyChristian R H Raetz
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Feb 25, 2006·The Journal of Biological Chemistry·Suparna Kanjilal-Kolar, Christian R H Raetz
Feb 10, 2006·The Journal of Biological Chemistry·Xiaoyuan WangChristian R H Raetz
Nov 6, 2008·The Journal of Biological Chemistry·Mark J KarbarzChristian R H Raetz
Mar 11, 2008·Applied and Environmental Microbiology·Hari B Krishnan, Demosthenis Chronis
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Dec 7, 2007·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·David M Byers, Huansheng Gong
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Jun 15, 2005·Annual Review of Biochemistry·Stephen W White Rock
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