Lipopolysaccharide enhances the transcription of prostaglandin H synthase-2 gene in primary human trophoblasts

American Journal of Obstetrics and Gynecology
E Y AntebyY Sadovsky

Abstract

Our purpose was to test the hypothesis that lipopolysaccharide potentiates the transcription of human placental prostaglandin H synthase-2 gene, a rate-limiting enzyme in prostaglandin synthesis. We transfected normal term cytotrophoblasts with a reporter vector containing either prostaglandin H synthase-1 or prostaglandin H synthase-2 promoters, cloned upstream of a luciferase. We correlated lipopolysaccharide effect on luciferase activity with enzyme expression and prostaglandin E2 production. Lipopolysaccharide (10 microg/ml) enhanced prostaglandin H synthase-2 but not prostaglandin H synthase-1 promoter activity. This effect was abolished by dexamethasone (10(-6) mol/L). Platelet-derived growth factor, a known stimulant of prostaglandin H synthase-2 in nonplacental tissue, had no effect on the activity of prostaglandin H synthase-2 promoter. Lipopolysaccharide induced the expression of prostaglandin H synthase-2 messenger ribonucleic acid fivefold to sixfold and caused a 3.5-fold increase in prostaglandin E2 production. Lipopolysaccharide enhances the transcription of prostaglandin H synthase-2 gene in human trophoblasts. Enhanced expression of prostaglandin H synthase-2 results in a higher production of prostaglandins, whi...Continue Reading

References

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Citations

Nov 28, 2006·Expert Opinion on Drug Metabolism & Toxicology·Margherita Strolin BenedettiEugène Baltes
Feb 15, 2001·American Journal of Obstetrics and Gynecology·G LeguizamónY Sadovsky
Jun 10, 2000·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·G GrossL J Muglia
Dec 20, 2003·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Tamsin Lindstrom, Phillip Bennett

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