Lipopolysaccharide Exposure Alleviates Asthma in Mice by Regulating Th1/Th2 and Treg/Th17 Balance

Medical Science Monitor : International Medical Journal of Experimental and Clinical Research
Fengxia DingBo Liu

Abstract

BACKGROUND It is generally believed that endotoxin exposure exacerbates risk of developing asthmatic symptoms. However, recent studies have indicated that prior bacterial exposure may prevent future symptoms of asthma. Here, we evaluated the influence of pre-exposure to different concentrations of lipopolysaccharide (LPS) to subsequent ovalbumin (OVA) allergen sensitization and challenge. MATERIAL AND METHODS Four-week-old Balb/c mice were treated intranasally with varying concentrations of LPS (1 ug, 10 ug, and 100 ug) or sterile PBS for 10 days, then 2 weeks later they were exposed to OVA. Both the molecular and functional airway responses to OVA administration were assessed following prior exposure to different doses of LPS or controls. Additionally, the Th1/Th2 and Treg/Th17 balance was measured. RESULTS Airway responsiveness and immune cell recruitment in the bronchoalveolar lavage (BALF) were decreased in animals exposed to a low dose of LPS (1 ug) treatment compared with the asthma group. Moderate-dose (10 ug) and high-dose (100 ug) LPS administration showed no differences from controls. Further, low-dose LPS (1 ug) exposure was associated with increased Th1 cytokines, T-bet, Treg cytokine (IL-10, TGF-β), and Foxp3 expre...Continue Reading

Citations

Nov 7, 2019·Current Opinion in Allergy and Clinical Immunology·Xuesong ChenEvan Li
Oct 23, 2019·Signal Transduction and Targeted Therapy·Seyyed Shamsadin Athari
Oct 18, 2020·Signal Transduction and Targeted Therapy·Seyyed Shamsadin Athari
Jun 11, 2021·Laboratory Investigation; a Journal of Technical Methods and Pathology·Xi ChenXuejun Guo
Jul 3, 2021·International Journal of Molecular Sciences·Marcello CandelliFrancesco Franceschi
Sep 21, 2021·Clinical Reviews in Allergy & Immunology·Luiz Otávio LourençoCarla Máximo Prado
Oct 31, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Xiangru ZhengYao Ouyang

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Methods Mentioned

BETA
PCR
ELISAs
flow cytometry

Software Mentioned

ImageJ2×
GraphPad Prism

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