PMID: 2098699Oct 1, 1990Paper

Lipopolysaccharide from Actinobacillus actinomycetemcomitans stimulates macrophages to produce interleukin-1 and tumor necrosis factor mRNA and protein

Oral Microbiology and Immunology
F R SaglieH P Koeffler

Abstract

Actinobacillus actinomycetemcomitans is associated with periodontal disease in children and adults. We report that low concentrations of lipopolysaccharide (LPS) from A. actinomycetemcomitans stimulated human macrophages to increase dramatically their accumulation of mRNA coding for interleukin-1 alpha (IL-1 alpha), IL-1 beta as well as tumor necrosis factor (TNF). Protein levels of IL-1 and TNF alpha also increased. Levels of these mRNAs increased by 4-5 fold as compared with unstimulated macrophages when these cells were cultured with as little as 2 ng/ml LPS from A. actinomycetemcomitans. Polymyxin binds and blocks the action of LPS; polymyxin inhibited the ability of LPS from A. actinomycetemcomitans to increase levels of IL-1 beta mRNA. The LPS of A. actinomycetemcomitans stimulated increased levels of IL-1 beta mRNA in the presence of cycloheximide, showing that stimulation by this LPS did not require new synthesis of protein. Furthermore, dexamethasone inhibited the ability of LPS from A. actinomycetemcomitans to stimulate the accumulation of mRNA coding for IL-1 beta. A. actinomycetemcomitans is an invasive microorganism of the gingiva; high intragingival numbers correlate with sites undergoing local destruction of the ...Continue Reading

References

Jun 3, 1977·Science·C R MundyJ G Bandelin
Jul 3, 1979·Calcified Tissue International·S L TeitelbaumA J Kahn
Oct 1, 1976·Immunochemistry·D C Morrison, D M Jacobs
Mar 3, 1978·Science·A J KahnS L Teitelbaum
Aug 26, 1985·Nucleic Acids Research·Y FurutaniS Nakamura
Feb 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·S W LeeA C Allison
Jan 1, 1985·Journal of Clinical Periodontology·J J Zambon
Mar 1, 1971·American Journal of Clinical Pathology·L T YamW H Crosby
Dec 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·P E AuronC A Dinarello
Sep 1, 1982·Journal of Periodontal Research·F R SaglieK J Lewin
Jul 1, 1983·Analytical Biochemistry·A P Feinberg, B Vogelstein
Mar 1, 1984·Journal of Dental Research·R J Genco, J Slots
Sep 1, 1984·Calcified Tissue International·T J Chambers, M A Horton
Oct 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·D A Goldberg

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Citations

Nov 1, 1993·Journal of Periodontology·M KjeldsenK Bendtzen
Nov 1, 1996·Annals of Periodontology·J J Zambon
May 1, 1991·Journal of Periodontal Research·R R Ranney
Jun 1, 1994·Periodontology 2000·A D Haffajee, S S Socransky
Jun 30, 2010·Infection and Immunity·Yu-Hsiung WangFrank C Nichols
Jun 10, 2017·Pharmacology Research & Perspectives·Tomonori FurukawaShinya Ueno
Oct 16, 1999·Periodontology 2000·P M Fives-TaylorC Brissette
Jan 1, 1997·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·D H MeyerP M Fives-Taylor
May 21, 2003·Periodontology 2000·Paul J Ezzo, Christopher W Cutler
Nov 26, 2003·Microbiology and Immunology·Tetsuyoshi InoueKazuhiro Fukui
Aug 1, 1996·Journal of Periodontal Research·M WilsonB Henderson
Nov 25, 1997·Journal of Cellular Physiology·S AmanoS Hanazawa

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