Lipopolysaccharide hyperpolarizes guinea pig airway epithelium by increasing the activities of the epithelial Na(+) channel and the Na(+)-K(+) pump

American Journal of Physiology. Lung Cellular and Molecular Physiology
Michael W Dodrill, Jeffrey S Fedan

Abstract

Earlier, we found that systemic administration of lipopolysaccharide (LPS; 4 mg/kg) hyperpolarized the transepithelial potential difference (V(t)) of tracheal epithelium in the isolated, perfused trachea (IPT) of the guinea pig 18 h after injection. As well, LPS increased the hyperpolarization component of the response to basolateral methacholine, and potentiated the epithelium-derived relaxing factor-mediated relaxation responses to hyperosmolar solutions applied to the apical membrane. We hypothesized that LPS stimulates the transepithelial movement of Na(+) via the epithelial sodium channel (ENaC)/Na(+)-K(+) pump axis, leading to hyperpolarization of V(t). LPS increased the V(t)-depolarizing response to amiloride (10 μM), i.e., offset the effect of LPS, indicating that Na(+) transport activity was increased. The functional activity of ENaC was measured in the IPT after short-circuiting the Na(+)-K(+) pump with basolateral amphotericin B (7.5 μM). LPS had no effect on the hyperpolarization response to apical trypsin (100 U/ml) in the Ussing chamber, indicating that channel-activating proteases are not involved in the LPS-induced activation of ENaC. To assess Na(+)-K(+) pump activity in the IPT, ENaC was short-circuited with a...Continue Reading

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Citations

Dec 14, 2011·American Journal of Physiology. Lung Cellular and Molecular Physiology·Preston GoodsonMy N Helms
Dec 2, 2011·Journal of Biomedicine & Biotechnology·Monika I HollenhorstMartin Fronius
Dec 20, 2016·Canadian Journal of Physiology and Pharmacology·Jia DengDa-Kai Xiang
Apr 8, 2020·Toxins·Rudolf LucasJürg Hamacher
Jun 11, 2014·Molecular Medicine Reports·Shu-Jing ShengWei-Wei Su
Jan 18, 2013·American Journal of Physiology. Cell Physiology·Paul M Vanhoutte

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