Lipopolysaccharide increased the expression levels of IL-18, ICE and IL-18 R in murine Leydig cells

American Journal of Reproductive Immunology : AJRI
Mahmoud AbuElhijaMahmoud Huleihel

Abstract

This study examined the effect of lipopolysaccharide (LPS) on the capacity of Leydig cells to produce and express interleukin-18 (IL-18), IL-18 receptor (IL-18R) and the IL-1beta-converting enzyme (ICE) (IL-18 family), under in vitro conditions. Primary Leydig cells (LCs) were isolated from murine testis by the Percoll technique, and cultured both in the presence and absence of LPS (0.1, 1, 5 microg/mL) for 3 and 24 hr. LCs were examined for their capacity to produce and express IL-18 family molecules by using immunohistochemical staining (IHC), enzyme-linked immunosorbent assay (ELISA), Western blot and real-time polymerase chain reaction (PCR) analysis. Leydig cells were shown to constitutively express IL-18, as examined by IHC, ELISA, Western blot and real-time PCR analysis. Addition of LPS to LC cultures was shown to significantly increase the basal levels of IL-18, in a dose- and time course-dependent manner, as examined by ELISA, Western blot and real-time PCR analysis. In addition, LPS increased LC cultures to express ICE and IL-18 R, as examined by real-time PCR analysis. Our results demonstrate that LPS increased the capacity of murine LCs to produce the IL-18 family molecules. IL-18, in the testis, might be involved i...Continue Reading

References

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Citations

Dec 3, 2008·American Journal of Reproductive Immunology : AJRI·Mahmoud Abu ElhijaMahmoud Huleihel
May 3, 2015·Reproduction : the Official Journal of the Society for the Study of Fertility·Taketo InoueMakoto Usami
May 28, 2011·Andrologia·A KomskyD Strassburger

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