Lipopolysaccharide increases monocyte binding to mesangial cells through fractalkine and its receptor

Transplantation Proceedings
J ParkH Ha

Abstract

Fractalkine (CX3CL1) is a unique chemokine that functions not only as a chemokine but also as an adhesion molecule. Fractalkine plays an important role in the recruitment of macrophages into the kidneys by binding to its specific receptor CX3CR1, and renal fractalkine expression was shown to be increased in chronic renal allograft rejection. Considering that microcapillary inflammation is a key feature of chronic renal allograft rejection, the present study examined whether monocytes bind to mesangial cells cultured in the presence of lipopolysaccharide (LPS) through fractalkine/CX3CR1 in order to understand their regulation with respect to inflammation-induced renal allograft dysfunction. Mouse mesangial cells were stimulated with LPS in the presence or absence of fractalkine or CX3CR1 siRNA. Calcein-AM-labeled monocytes were used to evaluate monocyte binding. Fractalkine and CX3CR1 mRNA and protein expression were measured by real-time quantitative polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. LPS at 100 ng/mL significantly increased monocyte binding to mesangial cells. Each siRNA against fractalkine or CX3CR1 effectively inhibited LPS-induced monocyte-mesangial cell binding. Fractalkine and C...Continue Reading

References

Mar 13, 2003·Kidney International·Ravinder S ChanaDavid C Wheeler
Mar 15, 2006·Transplantation·Brian J Nankivell, Jeremy R Chapman
Aug 1, 2006·The American Journal of Pathology·Kengo FuruichiPhilip M Murphy
Jun 21, 2007·Kidney International·M J KoziolekF Strutz
Dec 17, 2009·American Journal of Physiology. Heart and Circulatory Physiology·Li MengRama Natarajan

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