Lipopolysaccharide-induced neuroinflammation leads to the accumulation of ubiquitinated proteins and increases susceptibility to neurodegeneration induced by proteasome inhibition in rat hippocampus.

Journal of Neuroinflammation
Cristina PintadoDiego Ruano

Abstract

Neuroinflammation and protein accumulation are characteristic hallmarks of both normal aging and age-related neurodegenerative diseases. However, the relationship between these factors in neurodegenerative processes is poorly understood. We have previously shown that proteasome inhibition produced higher neurodegeneration in aged than in young rats, suggesting that other additional age-related events could be involved in neurodegeneration. We evaluated the role of lipopolysaccharide (LPS)-induced neuroinflammation as a potential synergic risk factor for hippocampal neurodegeneration induced by proteasome inhibition. Young male Wistar rats were injected with 1 μL of saline or LPS (5 mg/mL) into the hippocampus to evaluate the effect of LPS-induced neuroinflammation on protein homeostasis. The synergic effect of LPS and proteasome inhibition was analyzed in young rats that first received 1 μL of LPS and 24 h later 1 μL (5 mg/mL) of the proteasome inhibitor lactacystin. Animals were sacrificed at different times post-injection and hippocampi isolated and processed for gene expression analysis by real-time polymerase chain reaction; protein expression analysis by western blots; proteasome activity by fluorescence spectroscopy; immu...Continue Reading

References

Jun 1, 1995·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·R KieferH P Hartung
Apr 1, 1995·Current Opinion in Cell Biology·M Hochstrasser
Mar 9, 2000·Mechanisms of Ageing and Development·J N KellerW R Markesbery
Mar 27, 2001·Nature Reviews. Molecular Cell Biology·P M Kloetzel
Aug 28, 2001·Free Radical Biology & Medicine·Q Ding, J N Keller
Jan 18, 2005·Trends in Cell Biology·Martin Rechsteiner, Christopher P Hill
Jun 10, 2005·Proceedings of the National Academy of Sciences of the United States of America·Sylvia HeinkElke Krüger
Jun 21, 2005·Neurobiology of Aging·M Paz GavilánDiego Ruano
Nov 22, 2005·Neurobiology of Aging·Michele MishtoClaudio Franceschi
Jul 6, 2006·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Fumihiro ImaiMakoto Sawada
Sep 20, 2006·Journal of Neurochemistry·Rebecca GriffinMarina A Lynch
Sep 19, 2008·Expert Opinion on Drug Metabolism & Toxicology·Gaylia Jean Harry, Andrew D Kraft
Nov 7, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Sebastian JimenezJavier Vitorica
Nov 26, 2008·The International Journal of Biochemistry & Cell Biology·Frédéric EbsteinPeter-Michael Kloetzel
Feb 6, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Kate Lykke LambertsenBente Finsen
Apr 18, 2009·Molecular Aspects of Medicine·Tobias JungTilman Grune
Jun 17, 2010·Frontiers in Aging Neuroscience·Marina A Lynch
Jul 21, 2010·Acta Neuropathologica·V Hugh Perry
Apr 5, 2011·Biochemical and Biophysical Research Communications·Michele MishtoClaudio Franceschi
Aug 2, 2011·Journal of Neuroimmunology·Cristina PintadoAngélica Castaño
Aug 17, 2011·International Journal of Environmental Research and Public Health·Andrew D Kraft, G Jean Harry

❮ Previous
Next ❯

Citations

Jan 11, 2016·Annals of the New York Academy of Sciences·Maria E Figueiredo-PereiraJohn Babich
Jun 6, 2015·Brain, Behavior, and Immunity·Michele MishtoUwe Heinemann
May 23, 2015·Seminars in Immunopathology·Amma F AgyemangMichael F McDermott
Apr 19, 2015·Journal of Neuroinflammation·Amir NazemYousef Al-Abed
Aug 26, 2014·Frontiers in Molecular Neuroscience·Anne H P JansenElly M Hol
May 12, 2017·Alcoholism, Clinical and Experimental Research·Jorge MontesinosConsuelo Guerri
Nov 28, 2012·Nature Reviews. Neurology·Dimitrije Krstic, Irene Knuesel
Feb 7, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Laura de Diego GarcíaMiguel Díaz-Hernández
Aug 24, 2012·Journal of Neurochemistry·M Paz GavilánDiego Ruano
Apr 16, 2019·Frontiers in Immunology·Fiona LimanaqiFrancesco Fornai
May 28, 2019·Molecular Neurobiology·Sang-Gyun KangDavid Westaway
Aug 28, 2016·Journal of Neuroinflammation·J van ScheppingenE Aronica
Feb 27, 2016·Acta Neuropathologica Communications·Astrid SydowEva-Maria Mandelkow
Sep 14, 2018·Frontiers in Molecular Neuroscience·Fiona KerrOyinkan Sofola-Adesakin
Jan 19, 2021·European Journal of Pharmacology·Ranjit Singh, Ranju Bansal
Jul 21, 2020·Brain, Behavior, & Immunity Health·Gaia BrezzoChris Martin
Jul 6, 2021·Frontiers in Molecular Biosciences·Diego Ruano
Nov 2, 2019·Journal of Medicinal Chemistry·Breanna L ZerfasDarci J Trader

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR

Software Mentioned

Statgraphics plus
Applied

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.