Lipopolysaccharide induces bacterial autophagy in epithelial keratinocytes of the gingival sulcus.

BMC Cell Biology
Kanako Hagio-IzakiJun Ohno

Abstract

Interactions of resident bacteria and/or their producing lipopolysaccharide (LPS) with sulcular epithelial keratinocytes may be regulated by autophagy in the gingival sulcus. In this study, we investigated an induction of bacterial autophagy in exfoliative sulcular keratinocytes of the gingival sulcus and cultured keratinocytes treated with Porphyromonas gingivalis-originated LPS (PgLPS). Exfoliative sulcular keratinocytes showed an induction of autophagy, in addition to increased expression of LPS-mediated factors including lipopolysaccharide-binding protein and toll-like receptors (TLRs), leading to co-localization of bacteria with autophagosomes. In contrast, exfoliative keratinocytes from the free gingiva did not show similar autophagy. Autophagy activity in human cultured keratinocyte cells (HaCaT) was induced by PgLPS, which was dependent partially on the AMP-activated protein kinase (AMPK) pathway via increased intracellular reactive oxygen species (ROS) and was in association with an activation of TLR4 signaling. After incubation of cultured keratinocytes with E.coli BioParticles following PgLPS stimulation, co-localization of bioparticles with autophagosomes was enhanced. Conversely, blockage of autophagy with 3-methyl...Continue Reading

References

Feb 1, 1990·The Biochemical Journal·G A MurrellL Bromley
Oct 17, 1998·Journal of Clinical Microbiology·A L GriffenE J Leys
Mar 27, 2003·Periodontology 2000·Marja T PöllänenVeli-Jukka Uitto
Jun 23, 2004·Journal of Periodontal Research·Lei RenW Keung Leung
Oct 7, 2004·Applied and Environmental Microbiology·Daniel P GlavinJeffrey L Bada
Nov 6, 2004·Science·Ichiro NakagawaTamotsu Yoshimori
Oct 17, 2007·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Yoshihiko SawaShigemitsu Yoshida
Dec 7, 2007·Cell Metabolism·Cristina MammucariMarco Sandri
Feb 29, 2008·Nature·Noboru MizushimaDaniel J Klionsky
Dec 25, 2008·Annual Review of Immunology·Christian Münz
Dec 2, 2009·Cancer Biology & Therapy·Shi-Yong Sun
Feb 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Donna SirJing-Hsiung James Ou
Jun 22, 2010·Nature·Christian BehrendsJ Wade Harper
Sep 10, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Alexander DoyleYi-Ping Li
Dec 17, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hye-Mi LeeEun-Kyeong Jo
Jan 21, 2011·Nature·Beth LevineHerbert W Virgin
Jun 2, 2011·Molecular and Cellular Biology·Peter J Roach
Jul 20, 2011·Nature Chemical Biology·David CarlingSteven J Gamblin
Nov 15, 2011·Cell·Noboru Mizushima, Masaaki Komatsu
May 5, 2012·Trends in Cell Biology·Serge Mostowy, Pascale Cossart
Aug 31, 2012·Autophagy·Ivan TattoliStephen E Girardin
Feb 15, 2013·The New England Journal of Medicine·Augustine M K ChoiBeth Levine
Jul 20, 2014·Journal of the Formosan Medical Association = Taiwan Yi Zhi·Yen-Chun ChenChia-Chi Ku
May 27, 2016·International Journal of Medical Sciences·Moon-Soo KimJune-Ho Byun

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Citations

Jan 24, 2019·Oral Diseases·Ming JiangGuangxun Zhu
Feb 11, 2020·Klinicheskaia laboratornaia diagnostika·T M KaravaevaP P Tereshkov
Jul 6, 2021·Frontiers in Neuroscience·Arthur Sefiani, Cédric G Geoffroy

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Methods Mentioned

BETA
protein assay
fluorescence microscopy

Software Mentioned

STATVIEW

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