Lipopolysaccharide induces competence genes JE and KC in Balb/C 3T3 cells

Journal of Cellular Physiology
C S TannenbaumT A Hamilton

Abstract

The expression of the early genes JE and KC has been examined in Balb/C 3T3 cells treated with bacterial lipopolysaccharide (LPS). Previous studies showed that JE and KC mRNAs are induced in murine peritoneal macrophages treated with LPS, suggesting a role for these genes in inflammatory responses. Consistent with this possibility are recently published cDNA sequences which document that both genes are members of a superfamily of inflammation- and/or growth-related cytokines. In the present study, we provide evidence that the mRNAs for JE and KC are specifically induced by LPS treatment of Balb/c 3T3 cells. The LPS-stimulated expression of JE and KC was dose dependent, and exhibited a transient time course; message levels were maximal between 2 and 4 hr and declined by 8 hr. The LPS-augmented accumulation of JE and KC occurred even in the presence of cyclohexamide, which additionally had a superinducing effect on the expression of both genes. Cyclohexamide alone, in the absence of LPS, also induced JE and KC mRNA accumulation. LPS-stimulated JE and KC mRNA expression was dependent upon the stimulation of transcription as determined by nuclear "run-on" studies. Comparative analyses indicated that, under the conditions employed, ...Continue Reading

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Aug 10, 1999·Burns : Journal of the International Society for Burn Injuries·D E FaunceE J Kovacs
Jan 27, 2012·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·Tarek M El-Achkar, Xue-Ru Wu
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Oct 31, 1991·Biochemical and Biophysical Research Communications·S HanazawaS Kitano

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