Lipopolysaccharide interaction with hemolin, an insect member of the Ig-superfamily

FEBS Letters
S Daffre, I Faye

Abstract

This study is an attempt to reach some understanding of how insects recognize intruding microorganisms and foreign entities while executing an immune response. We used lipopolysaccharide (LPS) from Escherichia coli, bound to a radiolabeled iodinated crosslinker, to identify hemolymph proteins from the Hyalophora cecropia moth that have the capacity to bind LPS. High amounts of radioactivity were conferred to hemolin, an immunoglobulin and NCAM-related protein, the concentration of which increases in insect hemolymph upon bacterial infection. We could demonstrate a concentration-dependent binding of hemolin to LPS. In addition we could show that Lipid A can compete for this binding, whereas KDO has no effect, indicating that hemolin interacts specifically with the Lipid A moiety of LPS.

References

Oct 1, 1994·Insect Biochemistry and Molecular Biology·B J Blacklock, R O Ryan
May 1, 1993·Developmental and Comparative Immunology·O SchmidtS C Sun

❮ Previous
Next ❯

Citations

Oct 27, 1999·Archives of Insect Biochemistry and Physiology·X Q Yu, M R Kanost
May 29, 2003·Journal of Insect Physiology·Robert J. ZakarianManfred E. Rau
Jul 30, 1999·Developmental and Comparative Immunology·H L Mendoza, I Faye
Sep 12, 2002·Biochimica Et Biophysica Acta·Takashi Angata, Els Brinkman-Van der Linden
Apr 2, 1998·Current Opinion in Immunology·K Söderhäll, L Cerenius
Sep 13, 2002·Insect Biochemistry and Molecular Biology·X-Q YuM R Kanost
Sep 17, 2002·Insect Molecular Biology·K Roxström-LindquistI Faye
Dec 3, 1999·European Journal of Biochemistry·R BettencourtI Faye
Dec 24, 2010·The Journal of Biological Chemistry·Miryam Paola Alvarez-FloresAna Marisa Chudzinski-Tavassi
Feb 14, 1998·European Journal of Biochemistry·R BettencourtI Faye
Sep 12, 2009·Annual Review of Entomology·Otto SchmidtIngrid Faye
Nov 27, 2007·Developmental and Comparative Immunology·Lindsey S GarverGeorge Dimopoulos
Nov 30, 2006·Developmental and Comparative Immunology·Olle TereniusIngrid Faye
Nov 28, 2006·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Yanyuan BaoIsao Morishima
Jun 7, 2006·Journal of Insect Physiology·M Mahbubur RahmanOtto Schmidt
Nov 30, 2005·Insect Molecular Biology·K Roxström-LindquistI Faye
Mar 31, 2015·International Immunopharmacology·Yu SunChao-Liang Liu
May 11, 2016·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Ingrid Faye, Bo G Lindberg
Jul 30, 2016·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Rosemary Viola BoschAna Marisa Chudzinski-Tavassi
Aug 28, 2003·Archives of Insect Biochemistry and Physiology·Aristea MethenitiMaria Lambropoulou
May 5, 2018·Scientific Reports·Veeranarayanan Surya AathmanathanMuthukalingan Krishnan
Oct 7, 2020·The FEBS Journal·Catarina NunesTakashi Koyama

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.