Lipopolysaccharide needs soluble CD14 to interact with TLR4 in human monocytes depleted of membrane CD14

Microbes and Infection
Cristina MorenoAlfonso Sánchez-Ibarrola

Abstract

Toll-like receptors recognize specific patterns of microbial components and regulate the activation of both innate and adaptive immunity. TLR4 recognizes lipopolysaccharide (LPS) in monocytes/macrophages with the help of other molecules like CD14 and MD-2, which indicates that the functional LPS receptor forms a large complex. The functional relationship between the components has been the subject of debate, as have the modifications induced by the ligand in the expression of some of these components. Moreover, as for other members of this family of receptors, the possible direct interaction of receptors and their ligands is a matter of discussion. In this paper we address the question of whether the expression of some of the components influences the expression of the rest. Human monocytes in which CD14 has been downregulated through interference in the turnover of the molecule at the Golgi level, show normal membrane TLR4 expression, when compared with control cells. On the other hand, LPS alters membrane TLR4 expression by monocytes devoid of membrane CD14 only in the presence of human serum. The effect of serum is blocked by anti-CD14 monoclonal antibodies, which strongly suggests a functional role for soluble CD14/LPS comp...Continue Reading

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May 18, 2010·Nephrology·Nuria Garcia-FernandezIsabel Coma-Canella
Nov 12, 2010·Science Translational Medicine·Glenda TrujilloCory M Hogaboam
Apr 1, 2010·Journal of Inflammation·Alison L HartePhilip G McTernan
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Mar 14, 2020·Cell·Katherine A Fitzgerald, Jonathan C Kagan

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