Lipopolysaccharide of Legionella pneumophila shed in a liquid culture as a nonvesicular fraction arrests phagosome maturation in amoeba and monocytic host cells

FEMS Microbiology Letters
Eva M SeegerJürgen H Helbig

Abstract

After uptake by susceptible host cells, Legionella pneumophila displays the ability to arrest phagolysosome fusion. To elucidate the role of lipopolysaccharide (LPS) in this mechanism, we investigated its influence on Acanthamoeba castellanii, A/J mouse macrophages and human monocytes. For this, legionellae were cultured in broth to the replicative, noninfectious phase or to the infectious phase expressing virulence traits. Shed LPS-enriched outer membrane vesicles (OMV) and LPS species <300 kDa were obtained from L. pneumophila Corby strains possessing the virulence-associated LPS epitope recognized by monoclonal antibody (MAb) 3/1 and its mutant TF 3/1, which has lost this epitope due to a mutation in the lag-1 gene. The shed LPS components were attached by specific antibodies to latex beads and added to the host cells for phagocytosis. We demonstrated for the first time that evasion of lysosomal degradation of phagosomes for up to 5 h can also be set off by LPS that is not tied up in OMV. Moreover, our cell culture models showed that the influence of MAb 3/1-positive and -negative LPS was identical. Our data clearly substantiate that LPS is an independent factor for evading lysosomal degradation, which is independent of the ...Continue Reading

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Citations

Sep 27, 2012·Proceedings of the National Academy of Sciences of the United States of America·Ryan SimkovskyBianca Brahamsha
Sep 2, 2014·Pathogens and Disease·Marta Palusinska-SzyszWiesław I Gruszecki
Feb 28, 2016·Applied and Environmental Microbiology·Ryan SimkovskySusan S Golden
Aug 14, 2019·Infection and Immunity·Richard C WhiteNicholas P Cianciotto
Jan 11, 2020·Frontiers in Microbiology·Marta Palusinska-SzyszBozena Kowalczyk
Feb 6, 2021·International Journal of Molecular Sciences·Bozena KowalczykMarta Palusinska-Szysz
May 23, 2021·Advanced Drug Delivery Reviews·Anna Lena JungBirke J Benedikter

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