Lipopolysaccharide primes the respiratory burst of Atlantic salmon SHK-1 cells through protein kinase C-mediated phosphorylation of p47phox

Developmental and Comparative Immunology
Víctor H OlavarríaVictoriano Mulero

Abstract

The superoxide-producing NADPH oxidase complex of phagocytes plays a crucial role in host defenses against microbial infection. NADPH oxidase consists of a membrane heterodimeric protein, composed of gp91phox and p22phox, and the cytosolic proteins, p40phox, p47phox and p67phox. In the present study, we clone and sequence the full-length cDNAs coding for the Atlantic salmon (Salmo salar) phagocyte NADPH oxidase components, p47phox, p67phox and gp91phox, using a homology cloning approach. The sequences of these cDNAs showed that the S. salar p47phox, p67phox and gp91phox genes contained single open reading frames, which encoded predicted proteins of 413, 504 and 565 amino acids, respectively. Comparison of the deduced amino acid sequences showed that the S. salar p47phox, p67phox and gp91phox sequences shared 51, 45 and 68% identity with those of human components, respectively. Despite this relatively low homology between salmon and mammalian NADPH oxidase subunits, their functional domains are highly conserved. We also found that the mRNA levels of p47phox, p67phox and gp91phox expression were higher in immune-related tissues, such as kidney, spleen and gill. In addition, infection of the salmon macrophage cell line SHK-1 with ...Continue Reading

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Citations

Mar 17, 2012·Diseases of Aquatic Organisms·A J YañezJ G Carcamo
Aug 21, 2013·Developmental and Comparative Immunology·Leon GrayferMiodrag Belosevic
Dec 4, 2015·Biology·Jordan W HodgkinsonMiodrag Belosevic
Mar 19, 2011·Developmental and Comparative Immunology·Aja M Rieger, Daniel R Barreda
Dec 4, 2014·Molecular Immunology·Víctor H OlavarríaAlejandro Yáñez
Jul 3, 2016·Developmental and Comparative Immunology·Jimena CortésLuis Mercado
Oct 4, 2016·Expert Review of Vaccines·Kevin MaiseyMyron Christodoulides
Nov 13, 2015·Biology·Jeffrey J Havixbeck, Daniel R Barreda

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