Lipopolysaccharide protects polymorphonuclear leukocytes from apoptosis via tyrosine phosphorylation-dependent signal transduction pathways

The Journal of Surgical Research
J F SweeneyD B Hinshaw

Abstract

The present study was undertaken to determine if tyrosine phosphorylation signal transduction pathways, which are known to be activated in polymorphonuclear leukocytes (PMN) by lipopolysaccharide (LPS), play a role in priming of PMN oxidative burst and protection of PMN from apoptosis by LPS, and to determine if an interface between these two signaling pathways exists. PMN were combined with or without 10-fold serial dilutions (0.1 ng-1 microgram/ml) of LPS and incubated at 37 degrees C/5% CO2. After 24 h PMN apoptosis was assessed using fluorescence microscopy and DNA agarose gel electrophoresis. Additional PMN were pretreated with the tyrosine kinase inhibitors genistein and herbamycin A before addition of LPS. Tyrosine phosphorylation was detected by immunoblotting. Oxidant production was quantitated by following the oxidation of a chromophore to its fluorescent product. LPS delayed the onset of apoptosis and prolonged the survival of PMN in a dose-dependent fashion. Both tyrosine kinase inhibitors blocked the protective effect of LPS on PMN apoptosis; however, only genistein blocked the priming effect of LPS on PMN oxidative burst. Tyrosine phosphorylation signal transduction pathways are central to protection of PMN from a...Continue Reading

References

Nov 1, 1992·American Journal of Respiratory Cell and Molecular Biology·G CoxM Jordana
Aug 11, 1991·Nucleic Acids Research·P W LairdA Berns
Nov 8, 1994·Proceedings of the National Academy of Sciences of the United States of America·S YousefiH U Simon
Jun 20, 1995·Proceedings of the National Academy of Sciences of the United States of America·R A GottliebB M Babior
Apr 6, 1995·Biochemical and Biophysical Research Communications·S GasperiniM A Cassatella
Jul 1, 1993·The Journal of Clinical Investigation·M K WhyteC Haslett
Dec 26, 1995·Biochemical and Biophysical Research Communications·A G RossiC Haslett

❮ Previous
Next ❯

Citations

Jan 10, 2012·American Journal of Respiratory and Critical Care Medicine·Saskia BraberGert Folkerts
Jul 17, 2003·European Journal of Clinical Investigation·G CohenW H Hörl
Jul 15, 2003·Journal of Cellular Biochemistry·Yasuhiro TsukaharaPeter E Newburger
Nov 27, 2004·Research in Veterinary Science·Junichi SanoAtsuhiko Hasegawa
Jul 11, 2006·Lancet·K A BrownD F Treacher
Nov 17, 2004·Journal of Dairy Science·J MehrzadC Burvenich
Jul 29, 2010·Veterinary Research·Minh Hong Anh LeBaljit Singh
Sep 24, 2005·Transplantation·Franck JoucherMichele German-Fattal
Feb 7, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·J B KleinK R McLeish
May 8, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ian SabroeMoira K B Whyte
Sep 3, 2002·Respiratory Research·Vasanthi R SunilDebra L Laskin
May 22, 2003·International Immunopharmacology·Jonathan M LevittJohn F Sweeney

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis