Lipopolysaccharide Specific Immunochromatography Based Lateral Flow Assay for Serogroup Specific Diagnosis of Leptospirosis in India

PloS One
Shanmugam VanithamaniKalimuthusamy Natarajaseenivasan

Abstract

Leptospirosis is a re-emerging infectious disease that is under-recognized due to low-sensitivity and cumbersome serological tests. MAT is the gold standard test and it is the only serogroup specific test used till date. Rapid reliable alternative serogroup specific tests are needed for surveillance studies to identify locally circulating serogroups in the study area. In the present investigation the serological specificity of leptospiral lipopolysaccharides (LPS) was evaluated by enzyme linked immunosorbent assay (ELISA), dot blot assay and rapid immunochromatography based lateral flow assay (ICG-LFA). Sera samples from 120 MAT positive cases, 174 cases with febrile illness other than leptospirosis, and 121 seronegative healthy controls were evaluated for the diagnostic sensitivity and specificity of the developed assays. LPS was extracted from five locally predominant circulating serogroups including: Australis (27.5%), Autumnalis (11.7%), Ballum (25.8%), Grippotyphosa (12.5%), Pomona (10%) and were used as antigens in the diagnostics to detect IgM antibodies in patients' sera. The sensitivity observed by IgM ELISA and dot blot assay using various leptospiral LPS was >90% for homologous sera. Except for Ballum LPS, no other L...Continue Reading

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Citations

Jul 17, 2020·MSphere·Charles Solomon Akino MercyKalimuthusamy Natarajaseenivasan
Feb 3, 2021·Microbial Pathogenesis·Shanmugam VanithamaniKalimuthusamy Natarajaseenivasan

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Methods Mentioned

BETA
Enzyme linked immunosorbent assay
ELISA
Assay
Dot blot
ELISAs

Software Mentioned

Epi
SigmaPlot
graph pad prism

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