Lipopolysaccharide stabilizes the crystal packing of the ABC transporter MsbA

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Christopher L Reyes, Geoffrey Chang

Abstract

The ABC transporter MsbA is an integral membrane protein involved in the transport of lipid A and lipopolysaccharides to the outer leaflet of the inner membrane in bacteria. Here, the critical role of the natural substrate lipopolysaccharide in the crystallization and diffraction quality of MsbA crystals is reported. Initial crystals grown in complex with ATP-vanadate alone diffracted to approximately 9 A. Screening of the natural substrate lipopolysaccharides led to the crystallization of MsbA in complex with ADP-vanadate and Ra lipopolysaccharide. The increased order within the crystal lattice allowed structure determination to 4.2 A.

References

Oct 3, 1998·Acta Crystallographica. Section D, Biological Crystallography·A T BrüngerG L Warren
Dec 6, 2000·Microbiology and Molecular Biology Reviews : MMBR·M PutmanW N Konings
Mar 30, 2001·The Journal of Biological Chemistry·W T DoerrlerC R Raetz
Jun 5, 2002·Annual Review of Biochemistry·Christian R H Raetz, Chris Whitfield
Jul 18, 2002·The Journal of Biological Chemistry·William T Doerrler, Christian R H Raetz
Apr 19, 2003·Proceedings of the National Academy of Sciences of the United States of America·Huamin ZhangWilliam A Cramer
Jul 5, 2003·The Journal of Biological Chemistry·Galya ReuterHendrik W van Veen
Sep 29, 2004·Nature Structural & Molecular Biology·Christopher F Higgins, Kenneth J Linton
Nov 3, 2004·Biochimica Et Biophysica Acta·Anthony G Lee
May 14, 2005·Science·Amy L Davidson, Jue Chen

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Citations

Jun 5, 2007·The Journal of Membrane Biology·Milton H Saier
Oct 12, 2007·PLoS Biology·Peter P BorbatHassane S Mchaourab
Dec 31, 2008·Journal of Structural Biology·Andrew WardRonald A Milligan

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