Lipopolysaccharide stimulates expression of osteoprotegerin and receptor activator of NF-kappa B ligand in periodontal ligament fibroblasts through the induction of interleukin-1 beta and tumor necrosis factor-alpha

Bone
Naohisa WadaA Akamine

Abstract

Our recent work showed that human periodontal ligament fibroblasts (HPLF) secrete bioactive osteoprotegerin (OPG), which inhibits osteoclastic differentiation and activity. However, it is unknown how HPLF regulate bone metabolism in the presence of lipopolysaccharide (LPS), which is a cell component of gram-negative bacteria and a pathogen in inflammatory bone diseases such as periodontitis. The present study examined the effects of Escherichia coli LPS on the gene expression of interleukin-1 beta (IL-1 beta), tumor necrosis factor-alpha (TNF-alpha), OPG, and receptor activator of NF-kappa B ligand (RANKL) in HPLF using semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis. In HPLF cultured with LPS for 48 h, expression of both OPG and RANKL mRNA was up-regulated, whereas for up to 24 h of stimulation, such up-regulation was not observed. However, LPS increased expression of IL-1 beta and TNF-alpha mRNA within 6 h of treatment. Moreover, in HPLF cultured with IL-1 beta or TNF-alpha, OPG and RANKL expression was induced within 12 h of culture. The administration of neutralizing antibodies against human IL-1 beta or TNF-alpha to LPS-treated cultures of HPLF inhibited the induction of OPG and RANKL exp...Continue Reading

References

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Citations

Dec 17, 2008·Gene Therapy·J A CirelliW V Giannobile
Jan 1, 2010·Journal of Oral Microbiology·Mikihito KajiyaToshihisa Kawai
Aug 26, 2014·Archives of Oral Biology·Yingying WuLei Shu
Sep 6, 2013·Journal of Periodontology·Thawinee ChaichanasakulSotirios Tetradis
Apr 11, 2014·BioMed Research International·Bin ChenYin Xiao
Jan 28, 2014·Journal of Endodontics·Ildikó J Márton, Csongor Kiss
Aug 19, 2008·European Journal of Cell Biology·Penny Reid, Ingunn Holen
Jan 12, 2008·Progress in Lipid Research·Hisako HikijiSatoshi Ishii
Mar 21, 2007·Journal of Endodontics·David CoonJianing He
Jun 9, 2005·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Xiaochao WeiRegis J O'Keefe
Sep 25, 2009·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Anna C KrajewskiMichael J Noack
Jan 9, 2008·Journal of Cellular Physiology·Shinsuke FujiiAkifumi Akamine
Sep 2, 2008·Journal of Cellular Biochemistry·Junko IshiiSohei Kitazawa
Jan 10, 2006·Periodontology 2000·P Mark Bartold, A Sampath Narayanan
Nov 2, 2006·Journal of Periodontal Research·T YamamotoN Kanamura
Apr 11, 2012·Dental Traumatology : Official Publication of International Association for Dental Traumatology·Xuan ZhanEdith H Y Tong
Apr 20, 2012·Journal of Clinical Periodontology·Naohide YamamotoAkifumi Akamine
Oct 16, 2012·Journal of Cellular Physiology·Siobhan C GannonP Mark Bartold
Aug 18, 2010·Periodontology 2000·Brian HendersonDerren Ready

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