Lipopolysaccharide surface structure does not influence IcsA polarity

FEMS Microbiology Letters
Matthew Thomas DoyleRenato Morona

Abstract

Shigella species are the causative agents of human bacillary dysentery. These bacteria spread within the lining of the gut via a process termed actin-based motility whereby an actin 'tail' is formed at the bacterial pole. The bacterial outer membrane protein IcsA initiates this process, and crucially is precisely positioned on the bacterial polar surface. Lipopolysaccharide (LPS) O-antigen surface structure has been implicated as an augmenting factor of polarity maintenance due to the apparent dysregulation of IcsA polarity in O-antigen deficient strains. Due to Shigellae having long and short O-antigen chains on their surfaces, it has been proposed that O-antigen chain lengths are asymmetrically distributed to optimize IcsA exposure at the pole and mask exposure laterally. Additionally, it has been proposed that LPS O-antigen restricts IcsA diffusion from the pole by maintaining minimal membrane fluidity. This study utilizes minicells and quantitative microscopy providing data refuting the models of asymmetric masking and membrane diffusion, and supporting a model of symmetric masking of IcsA. We contend that IcsA surface distribution is equivalent between wild-type and O-antigen deficient strains, and that differences in cell...Continue Reading

References

Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·M AchtmanP Herrlich
May 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M L BernardiniP J Sansonetti
Jul 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·M B Goldberg, J A Theriot
Oct 1, 1996·Molecular Microbiology·R C SandlinA T Maurelli
Feb 1, 1997·Molecular Microbiology·L Van den BoschR Morona
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·K A Datsenko, B L Wanner
Aug 2, 2001·Proceedings of the National Academy of Sciences of the United States of America·M CharlesM B Goldberg
Sep 5, 2001·Molecular Microbiology·J R RobbinsJ A Theriot
Sep 22, 2001·Journal of Bacteriology·C M KoppelmanN Nanninga
May 3, 2003·FEMS Microbiology Letters·Renato Morona, Luisa Van Den Bosch
May 3, 2003·FEMS Microbiology Letters·Renato Morona, Luisa Van Den Bosch
Sep 3, 2004·Molecular Microbiology·Anthony P Pugsley, Nienke Buddelmeijer
Jul 15, 2009·Journal of Molecular Biology·Assaf RokneyAmos B Oppenheim
Jan 16, 2014·Microbiology·Elizabeth Ngoc Hoa TranRenato Morona
Jul 9, 2014·The Journal of Cell Biology·Anke Treuner-Lange, Lotte Søgaard-Andersen
Nov 14, 2014·Current Opinion in Gastroenterology·Ila F N LimaAldo A M Lima
Dec 4, 2014·Cellular Microbiology·Cesar M Valencia-GallardoGuy Tran Van Nhieu

❮ Previous
Next ❯

Citations

Sep 1, 2015·Microbiology·Matthew Thomas DoyleRenato Morona

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.