PMID: 7539837Mar 1, 1995Paper

Lipopolysaccharides from periodontal pathogens prime neutrophils for enhanced respiratory burst: differential effect of a synthetic lipid a precursor IVA (LA-14-PP)

Journal of Periodontal Research
Y AidaM J Pabst

Abstract

When neutrophils are incubated with bacterial lipopolysaccharide (LPS), they become primed for enhanced release of superoxide anion (O2-) in response to stimulation by FMLP. We investigated the human neutrophil-priming activity of LPS from the periodontal pathogens, Porphyromonas gingivalis (Pg), Prevotella intermedia (Pi) and Actinobacillus actinomycetemcomitans (Aa) in comparison with that of LPS from Escherichia coli (E. coli). The optimum conditions for LPS to prime neutrophils were assessed for every LPS and found to be as follows: Neutrophils were incubated with LPS in the presence of 10% heat-inactivated plasma and 1 mM EDTA at 37 degrees C for 30 min and then stimulated with 1 microM FMLP at 37 degrees C for 7 min. Under these conditions, half-maximum priming was observed at 6.2 ng/ml Pg-LPS, 45 ng/ml Pi-LPS, 1.5 ng/ml Aa-LPS and 1.5 ng/ml E. coli-LPS. The priming activity of each LPS was neutralized by polymyxin B. Anti-CD14 monoclonal antibody inhibited priming by all LPS. The priming by Aa-LPS and E. coli-LPS was inhibited by LA-14-PP, a synthetic lipid A precursor IVA, but that by Pg-LPS and Pi-LPS was not. Priming by tumor necrosis factor alpha was not affected by polymyxin B, anti-CD14 antibody or LA-14-PP. Gelati...Continue Reading

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Citations

Nov 22, 2011·International Immunopharmacology·Maria Elisa Drago-SerranoRafael Campos-Rodríguez
Sep 9, 2008·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Eric M LewisLinda C McPhail
Jun 21, 2017·International Immunopharmacology·Terukazu SanuiFusanori Nishimura
Nov 9, 2019·Pathogens·Georgios N BelibasakisAnders Johansson
Dec 15, 2004·Clinical Anatomy : Official Journal of the American Association of Clinical Anatomists & the British Association of Clinical Anatomists·Nelson Wood, Roger B Johnson
Nov 25, 2003·Journal of Periodontal Research·D EkuniT Watanabe

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