Lipopolysaccharides Improve Mesenchymal Stem Cell-Mediated Cardioprotection by MyD88 and stat3 Signaling in a Mouse Model of Cardiac Ischemia/Reperfusion Injury

Stem Cells and Development
Xiaona ChuYue Wang

Abstract

Bone marrow-derived mesenchymal stem cells (MSCs) improve cardiac function after ischemia/reperfusion injury, in part, due to the release of cytoprotective paracrine factors. Toll-like receptor 4 (TLR4) is expressed in MSCs and regulates the expression of cytoprotective factors, cytokines, and chemokines. Lipopolysaccharide (LPS) stimulation of TLR4 activates two distinct signaling pathways that are either MyD88 dependent or MyD88 independent/TIR-domain-containing adapter-inducing interferon-β (TRIF) dependent. While it was reported previously that LPS treatment improved MSC-mediated cardioprotection, the mechanism underlying such improved effect remains unknown. To study the role of MyD88 signaling in MSC cardioprotective activity, wild type (WT) and MyD88-/- MSCs were treated with LPS (200 ng/mL) for 24 h. WT and MyD88-/- MSCs with or without LPS pretreatment were infused into the coronary circulation of isolated mouse hearts (Langendorff model) and then subjected to ischemia (25 min) and reperfusion (50 min). Saline served as a negative control. Both untreated and LPS-pretreated WT MSCs significantly improved postischemic recovery of myocardial function of isolated mouse hearts, as evidenced by improved left ventricular deve...Continue Reading

References

Jun 20, 2003·The Journal of Biological Chemistry·Shaun K OlsenMoosa Mohammadi
Dec 9, 2004·International Immunology·Kiyoshi Takeda, Shizuo Akira
Feb 5, 2005·Circulation Research·Kai C Wollert, Helmut Drexler
Jul 9, 2005·Nature Biotechnology·Michael A Laflamme, Charles E Murry
Apr 4, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Massimiliano GnecchiVictor J Dzau
May 2, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Xinsheng ZhuWei Chao
May 24, 2006·Genomics·Yunlong LiuHoward J Edenberg
Sep 14, 2006·Current Opinion in Pediatrics·Ann Steele, Peter Steele
Sep 23, 2006·The New England Journal of Medicine·Birgit AssmusAndreas M Zeiher
Oct 14, 2006·Blood·Meirav Pevsner-FischerDov Zipori
May 15, 2007·Progress in Cardiovascular Diseases·Nicolas Christoforou, John D Gearhart
May 30, 2007·Archives of Internal Medicine·Ahmed Abdel-LatifBuddhadeb Dawn
Apr 4, 2008·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Troy A MarkelDaniel R Meldrum
Jul 16, 2008·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Nikolaos G Frangogiannis
Nov 26, 2008·Circulation Research·Massimiliano GnecchiVictor J Dzau
Feb 28, 2009·Nature Reviews. Drug Discovery·Andrew Beenken, Moosa Mohammadi
Mar 18, 2009·Bioinformatics·Cole TrapnellSteven L Salzberg
Nov 13, 2009·PloS One·Nils HomerStanley F Nelson
Apr 22, 2010·Heart Failure Reviews·Arash HaghikiaDenise Hilfiker-Kleiner
Mar 6, 2012·Cell Stem Cell·Sudhir H RanganathJeffrey M Karp
Nov 24, 2012·Human Molecular Genetics·Adrian G ToddElliot J Androphy
Feb 13, 2013·BMC Medical Genomics·Liran JuanYunlong Liu
Jul 3, 2013·Proceedings of the National Academy of Sciences of the United States of America·Andreas LinkermannStefan Krautwald
Feb 13, 2014·Cardiovascular Research·Arash HaghikiaDenise Hilfiker-Kleiner
May 22, 2016·Bioinformatics·Zuguang GuMatthias Schlesner

❮ Previous
Next ❯

Citations

Nov 7, 2019·International Journal of Molecular Sciences·Joan Oliva
Jan 17, 2020·Cardiovascular Pathology : the Official Journal of the Society for Cardiovascular Pathology·Kun-Sheng LiHai-Ying Li
Jul 27, 2021·Frontiers in Cellular and Infection Microbiology·Mohammad AqdasJaved Naim Agrewala
Aug 10, 2021·Journal of Molecular and Cellular Cardiology·Abraham L Bayer, Pilar Alcaide

❮ Previous
Next ❯

Datasets Mentioned

BETA
GSE64568

Methods Mentioned

BETA
Flow Cytometry
transfection
RNA-Seq
PCR
X-ray

Software Mentioned

SOLiD Experiment Tracking System
BFAST
SOLiD
SOLiD Instrument Control
edgeR
PowerLab
ImageJ
R package ComplexHeatmap

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.