Lipopolysaccharides induces MUC5AC overproduction in human nasal epithelium.

European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery
Weiwei WangLingyan Wan

Abstract

Hyperproduction of mucin in the nasal epithelium is an important feature of nasal inflammatory diseases. We investigated the mechanism of lipopolysaccharides (LPS) involvement in mucin 5 subtype AC (MUC5AC) expression in human nasal epithelial cells. The primary human nasal epithelial cells were cultured in vitro, which were treated with LPS (10 nM/ml or 1 μM/ml) for 12 and 24 h. LPS-induced MUC5AC protein was determined in nasal epithelial cells. The levels of nuclear factor kappa B p65 (NF-κBp65) and its inhibitor kappa Bα (IκBα) protein were also detected, and interleukin-1β (IL-1β) mRNA was detected by real-time PCR. LPS up-regulated MUC5AC protein in human nasal epithelial cells, and we determined that the up-regulation of MUC5AC expression was due to a time- and dose-dependent degradation of IκBα protein, which resulted in the increase of NF-κBp65 nuclear translocation. Subsequently, we also determined that LPS can induce IL-1β mRNA in a time- and dose-dependent manner. These data show that LPS treatment activated NF-κB by promoting the degradation of IκBα and the nuclear localization of NF-κBp65, which induced MUC5AC overproduction.

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Citations

Sep 13, 2013·BioMed Research International·Zipei LiuShuguang Wang
Feb 19, 2015·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Rui LongJuan Li
Jan 5, 2018·Laryngoscope Investigative Otolaryngology·Ahmed A Al-SayedEmad Massoud
Mar 18, 2015·American Journal of Rhinology & Allergy·Weiwei WangRuitao Zhang
May 17, 2014·The European Respiratory Journal·Erika TokitaBruce K Rubin
Mar 9, 2019·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·Wei Wei WangSheng Wen Shao
Apr 14, 2020·The Kaohsiung Journal of Medical Sciences·Yong-Ming ZhuJie-Yu Zhou
Apr 6, 2018·International Journal of Molecular Medicine·Lingling TangXiongbiao Wang
May 31, 2016·Stomatologii︠a︡·Т V Popruzhenko, S P Boris

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