Lipoprotein lipase increases lipoprotein binding to the artery wall and increases endothelial layer permeability by formation of lipolysis products

Circulation Research
J C RutledgeI J Goldberg

Abstract

Mechanisms responsible for the accumulation of low-density lipoprotein (LDL) were investigated in a new model, the perfused hamster aorta. To do this, we developed a method to study LDL flux in real time in individually perfused arteries; each artery served as its own control. Using quantitative fluorescence microscopy, the rates of LDL accumulation and efflux were separately determined. Perfusion of arteries with buffer plus lipoprotein lipase (LpL) increased LDL accumulation 5-fold (0.1 +/- 0.03 mV/min [control] versus 0.5 +/- 0.05 mV/min [LpL]) by increasing LDL retention in the artery wall. This effect was blocked by heparin and monoclonal antibodies directed against the amino-terminal region of apolipoprotein B (apo B). This suggests that specific regions of apo B are involved in LDL accumulation within arteries. Also, the effect of hydrolysis of triglyceride-rich lipoproteins on endothelial barrier function was studied. We compared endothelial layer permeability using a water-soluble reference molecule, fluorescently labeled dextran. When LpL was added to hypertriglyceridemic plasma, dextran accumulation within the artery wall increased > 4-fold (0.024 +/- 0.01 mV/min [control] versus 0.098 +/- 0.05 mV/min [LpL]). Under t...Continue Reading

References

Oct 1, 1992·The Journal of Clinical Investigation·S C RumseyI J Goldberg
Dec 1, 1992·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·L B NielsenK Kjeldsen
Nov 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·S Ylä-HerttualaJ L Witztum
Feb 1, 1992·The Journal of Clinical Investigation·U SaxenaI J Goldberg
Mar 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·U SaxenaI J Goldberg
Feb 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·S G YoungJ L Witztum
Nov 1, 1989·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·D C Schwenke, T E Carew
Dec 1, 1986·The Journal of Clinical Investigation·I J GoldbergH N Ginsberg
Sep 1, 1984·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·B HennigA A Spector
May 1, 1981·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·R E PitasR W Mahley
Feb 1, 1982·The Journal of Clinical Investigation·A ChaitJ D Brunzell
Mar 1, 1994·Atherosclerosis·F KarpeA Hamsten
Apr 1, 1994·Current Opinion in Lipidology·R J Havel
Sep 1, 1955·The Journal of Clinical Investigation·R J HAVELJ H BRAGDON

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Citations

Jan 11, 2012·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Alexander BarteltJoerg Heeren
Oct 4, 2003·Current Atherosclerosis Reports·Dianne HysonLars Berglund
Aug 7, 2004·Current Atherosclerosis Reports·Jila Kaberi Otarod, Ira J Goldberg
Mar 17, 2004·Current Atherosclerosis Reports·Mehmet Cilingiroglu, Christie Ballantyne
Jul 26, 2005·Atherosclerosis·Luis A CunibertiJosé P Werba
Jun 8, 2001·Journal of Controlled Release : Official Journal of the Controlled Release Society·L YuS Wan Kim
Apr 1, 1998·Trends in Cardiovascular Medicine·R C Leboeuf, S A Schreyer
May 15, 2001·Journal of the American College of Nutrition·B HennigC J McClain
Apr 29, 2000·Current Opinion in Lipidology·D J Rader, M Jaye
Jul 6, 2000·Current Opinion in Lipidology·I J GoldbergE P Lutz
Sep 28, 2002·Current Opinion in Lipidology·Karina Preiss-LandlRudolf Zechner
Jul 11, 2007·Current Opinion in Lipidology·Stephen G YoungAnne P Beigneux
May 20, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Limin WangJohn C Rutledge
Feb 10, 2009·Arteriosclerosis, Thrombosis, and Vascular Biology·Chuchun L ChangRichard J Deckelbaum
Oct 12, 2010·Arteriosclerosis, Thrombosis, and Vascular Biology·Chuchun L ChangRichard J Deckelbaum
Apr 30, 2011·Arteriosclerosis, Thrombosis, and Vascular Biology·Ira J GoldbergRuth McPherson
Jul 26, 2005·The Journal of Clinical Investigation·Toru SeoRichard J Deckelbaum
Nov 1, 2007·Nutrition & Metabolism·Amanda M Gonzales, Robert A Orlando
Sep 11, 2012·The Journal of Clinical Endocrinology and Metabolism·Lars BerglundUNKNOWN Endocrine society
May 1, 2006·Future Cardiology·Mikko P S Ares, Maria M Stollenwerk
Oct 18, 2011·Biochimica Et Biophysica Acta·Eric A Schwartz, Peter D Reaven
Jan 1, 2016·Trends in Pharmacological Sciences·Sivaram Pillarisetti
Aug 26, 1998·The American Journal of Medicine·R M Krauss
Nov 17, 2004·BMC Physiology·Lucyna NeugerGunilla Olivecrona
Aug 22, 2016·Progress in Cardiovascular Diseases·Nidhi AgrawalIra J Goldberg
May 6, 2008·Clinical Biochemistry·Nafila B Al Riyami, Jiri Frohlich
Dec 1, 2004·The Journal of Laboratory and Clinical Medicine·Ellen BrodinJohn-Bjarne Hansen
Jul 21, 2004·Trends in Cardiovascular Medicine·Uli C BroedlDaniel J Rader
Nov 28, 2002·Journal of Lipid Research·Martin MerkelIra J Goldberg
Aug 6, 2010·Clinical Science·Robin Altman, John C Rutledge
Feb 6, 2003·The Journal of Clinical Investigation·Hiroaki YagyuIra J Goldberg

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