Lipoprotein-mediated delivery of BODIPY-labeled sterol and sphingolipid analogs reveals lipid transport mechanisms in mammalian cells

Chemistry and Physics of Lipids
Elina Ikonen, Tomas Blom

Abstract

Lipids are often introduced into cell membranes directly from solvent or from lipophilic artificial carriers, such as cyclodextrins. A physiological lipid entry route into mammalian cells is via lipoprotein mediated uptake. In this review, we discuss the introduction of BODIPY-labeled sterol and sphingolipid analogs into mammalian cells via high- or low-density lipoproteins, and the novel findings made by using this strategy. Lipoprotein mediated delivery favors endocytic uptake and initial incorporation of the lipid into membranes of the endosomal compartments. This routing can therefore highlight physiological mechanisms of lipid entry into and exit from the endo-lysosomal membrane system. The underlying principles are of key importance for instance in controlling plasma cholesterol levels and in the development and regression of lysosomal lipid storage diseases. A common denominator for the BODIPY-labeled lipid analogs discussed in this review is that they were synthesized by late Robert Bittman, whose scientific impact radiates far beyond his lifework in organic chemistry.

References

Oct 2, 2007·Journal of Lipid Research·Per HaberkantBritta Brügger
Oct 5, 2007·The Journal of Organic Chemistry·Zaiguo Li, Robert Bittman
Jan 25, 2008·Nature Reviews. Molecular Cell Biology·Elina Ikonen
Mar 3, 2009·Biochemical and Biophysical Research Communications·Padmavathi BandhuvulaJulie D Saba
Oct 1, 2011·Journal of Lipid Research·Sandhya SankaranarayananGeorge H Rothblat
Jan 18, 2013·PLoS Pathogens·Jameson T CrowleyJorge L Benach
Mar 26, 2013·The Journal of Clinical Investigation·Catherine MartelGwendalyn J Randolph
Jul 3, 2013·Journal of Cell Science·Vilja PietiäinenElina Ikonen
Jan 5, 2014·PloS One·Kaemisa SrisenJosef Neumüller
Apr 2, 2015·The Journal of Clinical Investigation·Richard L Proia, Timothy Hla
Aug 19, 2015·Nature Chemical Biology·Tomas BlomElina Ikonen

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Citations

Oct 30, 2016·Journal of Investigative Medicine : the Official Publication of the American Federation for Clinical Research·Cuiwen HeHaibo Jiang
Jan 1, 2015·Lipid Insights·Katarzyna A SolankoDaniel Wüstner
Aug 6, 2019·The Journal of Physical Chemistry. B·Peter ReinholdtJacob Kongsted

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