Lipoprotein profiles in SCID/uPA mice transplanted with human hepatocytes become human-like and correlate with HCV infection success

American Journal of Physiology. Gastrointestinal and Liver Physiology
Rineke H G SteenbergenNorman M Kneteman

Abstract

Although multiple determinants for hepatitis C virus (HCV) infection are known, it remains partly unclear what determines the human specificity of HCV infection. Presumably, the presence of appropriate entry receptors is essential, and this may explain why HCV is unable to infect nonhuman hepatocytes. However, using mice with chimeric human livers, we show in this study that the presence of human hepatocytes, and therefore human entry receptors, is not sufficient for HCV infection. In successfully transplanted SCID/Alb-uPA mice, infection with HCV is reliable only when ∼70-80% of the liver consists of human hepatocytes. We show that chimeric mice, which are hard to infect with HCV, have significant groups of human hepatocytes that are readily infected with hepatitis B virus. Thus it is unlikely that the lack of infection with HCV can simply be attributed to low hepatocyte numbers. We investigated whether the humanization of lipoprotein profiles is positively associated with infection success. We show that the lipoprotein profiles of chimeric mice become more human-like at high levels of engraftment of human hepatocytes. This and expression of markers of human lipoprotein biosynthesis, human apolipoprotein B (ApoB) and cholester...Continue Reading

References

Jan 1, 1992·Medical Microbiology and Immunology·R ThomssenA Uy
Sep 1, 1991·Journal of Clinical Microbiology·R BoomJ van der Noordaa
Aug 2, 2001·Nature Medicine·D F MercerN M Kneteman
Jul 26, 2002·Medical Microbiology and Immunology·Reiner Thomssen, Sigrid Bonk
Mar 26, 2005·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Philip MeulemanGeert Leroux-Roels
Jan 31, 2006·International Journal for Parasitology·John B SacciNorman M Kneteman
Oct 31, 2006·Virology Journal·Susan L UprichardTakaji Wakita
May 23, 2007·Cellular Microbiology·Ursula AndréoAgata Budkowska
Aug 1, 2007·Nature Biotechnology·Hisaya AzumaMarkus Grompe
Dec 14, 2007·Proceedings of the National Academy of Sciences of the United States of America·Karl-Dimiter BissigInder M Verma
Jan 8, 2008·The Journal of Biological Chemistry·Carlos MinahkDennis E Vance
Aug 19, 2008·Antiviral Research·Philip Meuleman, Geert Leroux-Roels
Mar 7, 2009·The Journal of General Virology·Michela E Burlone, Agata Budkowska

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Citations

Nov 1, 2012·Seminars in Immunopathology·M F BassendineR D G Neely
Jun 28, 2012·World Journal of Gastroenterology : WJG·Kristin L MacArthurGeorge Y Wu
Mar 31, 2015·European Journal of Pharmacology·Donna N Douglas, Norman M Kneteman
Apr 25, 2012·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Kazuaki ChayamaMichio Imamura
Jun 19, 2013·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Rineke H G SteenbergenD Lorne Tyrrell
Dec 11, 2013·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·John R FosterNorman M Kneteman
Jul 2, 2011·Journal of Hepatology·Margaret F BassendineR Dermot G Neely
Jun 25, 2016·Viruses·Ahmed Atef MesalamPhilip Meuleman
Sep 22, 2015·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·D O'SheaN Kneteman
Jun 20, 2019·Cells·Kylie Su Mei YongQingfeng Chen
Dec 18, 2019·Cold Spring Harbor Perspectives in Medicine·Alexander Ploss, Amit Kapoor
Feb 16, 2016·World Journal of Gastroenterology : WJG·Germana GrassiTonino Alonzi
Jun 24, 2021·Scientific Reports·Fereshte DamavandiGane Ka-Shu Wong

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