Abstract
1. Human plasma contains unidentified components that inhibit arachidonic acid (AA) metabolism. In the present study, we investigated whether plasma from rabbits fed a normal or high-cholesterol diet for 16 weeks also inhibits AA metabolism. Specifically, we studied the effects of plasma on platelet aggregation and on the production of AA metabolites, tri-hydroxyeicosatrienoic acid, 12-hydroxyeicosatetraenoic acid and thromboxane B(2). 2. Haematological and lipid profiles were altered by a high-cholesterol diet. Platelets from hypercholesterolaemic rabbits showed enhanced aggregatory sensitivity to AA and platelet-activating factor. However, plasma from hypercholesterolaemic and control rabbits, when added to the incubation mixture, significantly inhibited platelet aggregation and eicosanoid production. 3. High- and low-density lipoprotein (HDL and LDL, respectively) concentrations increased several-fold in plasma with cholesterol feeding. When added directly to the incubation mixture, both HDL and LDL inhibited platelet aggregation, as well as AA metabolism. 4. Haptoglobin, albumin and Cohn's fraction IV, but not globulins, exhibited antiplatelet and anti-AA metabolism activities. Their concentrations in plasma were not affect...Continue Reading
References
Jan 1, 1979·Lancet·E TremoliC Galli
Nov 3, 1977·Nature·S A SaeedH O Collier
May 1, 1992·Circulation·G DavìC Patrono
Aug 1, 1990·European Heart Journal·E B Smith
Feb 17, 1986·Life Sciences·P J Conn, E Sanders-Bush
Jun 1, 1988·Thrombosis Research·D PriscoG G Neri Serneri
Mar 1, 1986·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·G DiMinnoM Mancini
Feb 1, 1986·Atherosclerosis·W T JohnsonO Horwitz
Feb 21, 1974·The New England Journal of Medicine·A C CarvalhoR S Lees
Oct 15, 1983·The Biochemical Journal·D G HassallK R Bruckdorfer
Oct 1, 1982·The Journal of Clinical Endocrinology and Metabolism·S A SaeedM D Mitchell
Apr 15, 1982·Thrombosis Research·M Aviram, G J Brook
Jan 3, 1980·The New England Journal of Medicine·M J StuartJ G White
Dec 1, 1995·Circulation·L LacosteD Waters
Jan 1, 1995·Arteriosclerosis, Thrombosis, and Vascular Biology·A BröijersénP Hjemdahl
Aug 1, 1993·The Journal of Pharmacy and Pharmacology·S A SaeedN N Rahman
Jan 1, 1993·Seminars in Thrombosis and Hemostasis·E TremoliG Di Minno
Jul 1, 1997·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·S A SaeedB H Shah
Aug 22, 2002·Platelets·Derya OzsavciEkin Onder
Nov 2, 2002·Nature Medicine·Daniel Steinberg
Jun 27, 2003·Cellular and Molecular Life Sciences : CMLS·I A M RelouE Malle
Jul 23, 2003·Journal of Thrombosis and Haemostasis : JTH·S GrgurevichL K Jennings
Oct 29, 2005·Clinical Biochemistry·Azize SenerK Turay Yardimci
Jan 3, 2007·Biochemical and Biophysical Research Communications·Sheikh Arshad SaeedSagheer Ahmed
May 8, 2007·Prostaglandins & Other Lipid Mediators·Magnus BäckSven-Erik Dahlén
Citations
Aug 5, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sagheer AhmedMarius Moga
Nov 28, 2014·Journal of Translational Medicine·Saima GulMuhammad Zia-Ul-Haq
Feb 14, 2016·Analytical and Bioanalytical Chemistry·Zheng XiangDahui Chen
Jul 7, 2011·Current Opinion in Hematology·Craig N Morrell, Sanjay B Maggirwar