PMID: 15383598Sep 24, 2004Paper

Lipoproteins, not lipopolysaccharide, are the key mediators of the proinflammatory response elicited by heat-killed Brucella abortus

The Journal of Immunology : Official Journal of the American Association of Immunologists
Guillermo H GiambartolomeiM T Philipp

Abstract

Inflammation is a hallmark of brucellosis. Although Brucella abortus, one of the disease's etiologic agents, possesses cytokine-stimulatory properties, the mechanism by which this bacterium triggers a proinflammatory response is not known. We examined the mechanism whereby heat-killed B. abortus (HKBA), as well as its LPS, induces production of inflammatory cytokines in monocytes/macrophages. Polymyxin B, a specific inhibitor of LPS activity, did not inhibit the production of TNF-alpha- and IL-6-induced HKBA in the human monocytic cell line THP-1. HKBA induced the production of these cytokines in peritoneal macrophages of both C3H/HeJ and C3H/HeN mice, whereas B. abortus LPS only stimulated cells from C3H/HeN mice. Anti-TLR2 Ab, but not anti-TLR4 Ab, blocked HKBA-mediated TNF-alpha and IL-6 production in THP-1 cells. Because bacterial lipoproteins, a TLR2 ligand, have potent inherent stimulatory properties, we investigated the capacity of two B. abortus lipoproteins, outer membrane protein 19 (Omp19) and Omp16, to elicit a proinflammatory response. Lipidated (L)-Omp16 and L-Omp19, but not their unlipidated forms, induced the secretion of TNF-alpha, IL-6, IL-10, and IL-12 in a time- and dose-dependent fashion. Preincubation of T...Continue Reading

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Citations

Nov 1, 2008·Journal of Periodontal Research·G L Torres de HeensB G Loos
Nov 15, 2011·Journal of Leukocyte Biology·M Victoria DelpinoGuillermo H Giambartolomei
Mar 6, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Marco Tulio R GomesSergio C Oliveira
Apr 13, 2013·Scandinavian Journal of Immunology·E Eskandari-NasabH Pourmasoumi
May 6, 2015·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Morteza AbkarMehdi Kamali
Jul 14, 2016·Seminars in Immunopathology·Fernanda M MarimSergio C Oliveira
Apr 12, 2017·Glia·Ana M RodríguezGuillermo H Giambartolomei
Oct 17, 2017·PloS One·Andrea G FernándezPablo C Baldi
Jun 6, 2018·Frontiers in Immunology·Aldana TrottaPaula Barrionuevo
May 2, 2018·Japanese Journal of Infectious Diseases·Young Bin ImHan Sang Yoo
Feb 11, 2020·Frontiers in Immunology·Paula Constanza Arriola BenitezMaría Victoria Delpino
Jan 8, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Gilson Costa MacedoSergio Costa Oliveira
Sep 14, 2007·Scandinavian Journal of Infectious Diseases·Hasan KarsenMahmut Sünnetçioğlu
Aug 17, 2011·Infection and Immunity·Fernanda S OliveiraSérgio C Oliveira
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Jan 21, 2020·Frontiers in Cellular and Infection Microbiology·Guillermo Hernán Giambartolomei, María Victoria Delpino
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Sep 17, 2013·Microbiology and Immunology·Ebrahim Eskandari-NasabMehran Ghazali-bina

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