Liposomal Curcumin Targeting Endometrial Cancer Through the NF-κB Pathway

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Hanzi XuJinhai Tang

Abstract

Emerging evidence suggests that curcumin possesses chemopreventive properties against various cancers. However, its poor bioavailability limits its clinical application. In this study, we aimed to utilize encapsulation in liposomes (Lipo) as a strategy for the clinical administration of curcumin for endometrial carcinoma (EC). Curcumin was encapsulated in a liposomal delivery system to prepare a formulation of liposomal curcumin (LC). EC cell lines Ishikawa and HEC-1 were treated with the compound and cell proliferation was measured using MTT assay. Hoechst 33258 staining assay and flow cytometry were used to detect apoptosis of the cells. Wound healing and cell invasion assays were employed to monitor cell motility. Underlying target signaling, such as NF-κB, caspases, and MMPs, were further studied via qRT-PCR and western blot. Thereafter, a zebrafish model was used to assess the toxicity of LC. Finally, a zebrafish transplantation tumor model of EC was grown and treated with LC. Tumors were monitored and harvested to study the expression of NF-κB. The formation of LC was successfully developed with excellent purity and physical properties. In vitro, LC resulted in dose-dependent inhibition of proliferation, induction of apop...Continue Reading

Citations

Dec 31, 2019·Pharmaceutical Frontiers·Max KullbergHolly A Martinson
Mar 19, 2020·Diabetes, Metabolic Syndrome and Obesity : Targets and Therapy·Pingping ZhangDongmei Gan
Jun 7, 2020·Cell Biochemistry and Biophysics·Fisayo OlotuMahmoud E S Soliman
Feb 4, 2021·International Journal of Molecular Sciences·Marta WoźniakSiddarth Agrawal
Nov 6, 2020·Journal of Ovarian Research·Mohammad Hossein PourhanifehHamed Mirzaei
Feb 20, 2021·Cancer Cell International·Fahime JahanbakhshiJamal Halajzadeh
Mar 31, 2021·International Journal of Pharmaceutics·Natália A D'AngeloAndré M Lopes
Aug 28, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Tarek BenameurChiara Porro
Sep 22, 2021·Advances in Skin & Wound Care·Pushkar Satish SatheLaura L Bolton
Nov 5, 2020·Recent Patents on Anti-cancer Drug Discovery·Saleh A AlmatroodiArshad Husain Rahmani

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Apoptotic Caspases

Apoptotic caspases belong to the protease enzyme family and are known to play an essential role in inflammation and programmed cell death. Here is the latest research.

Breast Cancer: Chemo-Resistance

Some cancers are difficult to treat and aggressive including the "triple-negative" breast cancer. This type of cancer is chemoresistant even before chemotherapy begins. Here are the latest discoveries chemo-resistance in breast cancer.