Liposomal delivery of p-ialB and p-omp25 DNA vaccines improves immunogenicity but fails to provide full protection against B. melitensis challenge.

Genetic Vaccines and Therapy
Nicola J CommanderJudith A Stack

Abstract

We have previously demonstrated protective efficacy against B. melitensis using formulations of naked DNA vaccines encoding genes ialB and omp25. The present study was undertaken to further understand the immune response generated by the protective vaccination regimens and to evaluate cationic liposome adsorption as a delivery method to improve vaccine utility. The protective efficacy and immunogenicity of vaccines delivered as four doses of naked DNA, a single dose of naked DNA or a single dose of DNA surface adsorbed to cationic liposomes were compared using the BALB/c murine infection model of B. melitensis. Antigen-specific T cells and antibody responses were compared between the various formulations. The four dose vaccination strategy was confirmed to be protective against B. melitensis challenge. The immune response elicited by the various vaccines was found to be dependent upon both the antigen and the delivery strategy, with the IalB antigen favouring CD4+ T cell priming and Omp25 antigen favouring CD8+. Delivery of the p-ialB construct as a lipoplex improved antibody generation in comparison to the equivalent quantity of naked DNA. Delivery of p-omp25 as a lipoplex altered the profile of responsive T cells from CD8+ to...Continue Reading

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Citations

Apr 1, 2011·Drug Delivery and Translational Research·Deepika JainRanjit Singh
Jul 26, 2012·Expert Review of Vaccines·Karen Smith KorsholmDennis Christensen
Nov 8, 2014·Clinical and Vaccine Immunology : CVI·Sarah Mackenzie-DyckSylvia van Drunen Littel-van den Hurk
Nov 16, 2016·PloS One·Tatiane F CarvalhoRenato L Santos

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Methods Mentioned

BETA
PCR
transfection
electrophoresis
ELISA

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