Liposomal Formulations for an Efficient Encapsulation of Epigallocatechin-3-gallate: An in- Silico/Experimental Approach

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Emiliano LaudadioRoberta Galeazzi

Abstract

As a part of research project aimed to optimize antioxidant delivery, here we studied the influence of both salts and lipid matrix composition on the interaction of epigallocatechin-3-gallate (EGCG) with bilayer leaflets. Thus, we combined in silico and experimental methods to study the ability of neutral and anionic vesicles to encapsulate EGCG in the presence of Ca2+ and Mg2+ divalent salts. Experimental and in silico results show a very high correlation, thus confirming the efficiency of the developed methodology. In particular, we found out that the presence of calcium ions hinders the insertion of EGCG in the liposome bilayer in both neutral and anionic systems. On the contrary, the presence of MgCl₂ improves the insertion degree of EGCG molecules respect to the liposomes without divalent salts. The best and most efficient salt concentration is that corresponding to a 5:1 molar ratio between Mg2+ and EGCG, in both neutral and anionic vesicles. Concerning the lipid matrix composition, the anionic one results in better promotion of the catechin insertion within the bilayer since experimentally we achieved 100% EGCG encapsulation in the lipid carrier in the presence of a 5:1 molar ratio of magnesium. Thus, the combination of ...Continue Reading

References

Sep 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·F Szoka, D Papahadjopoulos
Aug 5, 2000·Archives of Dermatology·S K KatiyarH Mukhtar
Mar 10, 2001·BioFactors·T NakayamaS Kumazawa
Jan 25, 2002·Annual Review of Pharmacology and Toxicology·Chung S YangXiaofeng Meng
Feb 6, 2002·Bioscience, Biotechnology, and Biochemistry·K KajiyaT Nakayama
Jun 6, 2002·Chemistry and Physics of Lipids·Hans Binder, Olaf Zschörnig
Jul 21, 2004·Journal of Computational Chemistry·Eric F PettersenThomas E Ferrin
Nov 16, 2004·International Journal of Pharmaceutics·Li ZhangZhong Zuo
Feb 3, 2005·Nature Reviews. Drug Discovery·Vladimir P Torchilin
Mar 5, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·S Moein MoghimiJ Clifford Murray
Sep 20, 2005·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·Chung-Hwan ChenGwo-Jaw Wang
Oct 4, 2005·Journal of Computational Chemistry·David A CaseRobert J Woods
Feb 21, 2006·Journal of Controlled Release : Official Journal of the Controlled Release Society·A LayreR Gref
Oct 13, 2006·Nature Biotechnology·Volker WagnerAxel Zweck
Oct 18, 2006·The Journal of Chemical Physics·Jeffery B KlaudaRichard W Pastor
Oct 31, 2006·Advanced Drug Delivery Reviews·Omid C Farokhzad, Robert Langer
Mar 22, 2007·Journal of Agricultural and Food Chemistry·Minghui FanXiaoming Zhang
May 19, 2007·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·A Migalska-ZalasB Sahraoui
Jun 12, 2009·Medicine and Science in Sports and Exercise·David C NiemanThomas C Lines
May 26, 2010·The Journal of Physical Chemistry. B·Jeffery B KlaudaRichard W Pastor
Aug 12, 2010·The Journal of Membrane Biology·Jan DomańskiOliver Beckstein
Oct 7, 2010·The Journal of Physical Chemistry. B·Sunhwan JoWonpil Im
Feb 8, 2011·Biochemistry·John M BoettcherChad M Rienstra
Mar 5, 2011·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Shengmin SangChung S Yang
May 3, 2011·The Journal of Chemical Physics·Alexey V Onufriev, Grigori Sigalov
Jul 25, 2012·Biophysical Journal·Alberto Martín-MolinaJordi Faraudo
Apr 23, 2013·Journal of Liposome Research·Rita Castillo Cohen de PaceShu Wang
Jul 9, 2013·Journal of Computational Chemistry·Jing Huang, Alexander D MacKerell
Sep 4, 2013·Journal of Computational Chemistry·Christopher G MayneJames C Gumbart
Oct 26, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Diana Y VillanuevaJeffery B Klauda
Mar 20, 2015·Drug Delivery and Translational Research·Maria-Lucia BriugliaDimitrios A Lamprou
Nov 4, 2015·Biochemical and Biophysical Research Communications·Lingxue MaoHuaiyu Yang
Jan 16, 2016·Current Drug Delivery·Marjan DaeihamedMasoud Faghih Akhlaghi
May 24, 2016·Nutrients·Andreia GranjaSalette Reis
Dec 3, 2016·Scientific Reports·Adéla MelcrováLukasz Cwiklik
Jun 22, 2017·Molecular Informatics·Emiliano LaudadioRoberta Galeazzi

❮ Previous
Next ❯

Methods Mentioned

BETA
Dynamic Light Scattering

Software Mentioned

CHIMERA
Maestro
CHARMM
Macromodel
G09 of Gaussian
VMD
Xleap
cluster
GUI
GROMACS

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.