Liposome-based polymer complex as a novel adjuvant: enhancement of specific antibody production and isotype switch.

International Journal of Nanomedicine
Chia-Hung ChenKuang-Wen Liao

Abstract

The aim of vaccination is to induce appropriate immunity against pathogens. Antibody-mediated immunity is critical for protection against many virus diseases, although it is becoming more evident that coordinated, multifunctional immune responses lead to the most effective defense. Specific antibody (Ab) isotypes are more efficient at protecting against pathogen invasion in different locations in the body. For example, compared to other Ab isotypes, immunoglobulin (Ig) A provides more protection at mucosal areas. In this study, we developed a cationic lipopolymer (liposome-polyethylene glycol-polyethyleneimine complex [LPPC]) adjuvant that strongly adsorbs antigens or immunomodulators onto its surface to enhance or switch immune responses. The results demonstrate that LPPC enhances uptake ability, surface marker expression, proinflammatory cytokine release, and antigen presentation in mouse phagocytes. In contrast to Freund's adjuvant, LPPC preferentially activates Th1-immunity against antigens in vivo. With lipopolysaccharides or CpG oligodeoxynucleotides, LPPC dramatically enhances the IgA or IgG2A proportion of total Ig, even in hosts that have developed Th2 immunities and high IgG1 serum titers. Taken together, the results ...Continue Reading

References

Apr 1, 1996·Current Opinion in Immunology·J Stavnezer
Oct 31, 1996·Nature·A K AbbasA Sher
Jul 30, 1999·Journal of Controlled Release : Official Journal of the Controlled Release Society·W T GodbeyA G Mikos
Dec 29, 2000·Nature·H HemmiS Akira
Aug 30, 2002·Molecular Immunology·Monique Grommé, Jacques Neefjes
Feb 6, 2003·The American Journal of Chinese Medicine·Chian-Jiun Liou, Jerming Tseng
May 25, 2004·The American Journal of Chinese Medicine·Chian-Jiun LiouJerming Tseng
Nov 16, 2004·The Journal of Gene Medicine·Akin AkincRobert Langer
Jul 9, 2005·Journal of Biochemistry·Midori KitazoeHidenori Yamada
Feb 17, 2007·Immunology·Karen Smith KorsholmPeter Andersen
Feb 27, 2007·Journal of Zhejiang University. Science. B·Zahid Iqbal RajputAbdullah G Arijo
Mar 6, 2007·Vaccine·J C Aguilar, E G Rodríguez
Sep 28, 2007·British Journal of Haematology·Anne F McGettrick, Luke A J O'Neill
Jun 12, 2009·Journal of Liposome Research·Dennis ChristensenYvonne Perrie
Nov 26, 2009·Molecular Therapy : the Journal of the American Society of Gene Therapy·Christian MuellerTerence R Flotte
Oct 23, 2010·Biomaterials·Michael J HeffernanJohn W Greiner
Feb 4, 2011·International Journal of Nanomedicine·Jian ChenYuhong Xu
Mar 18, 2011·Journal of Medical Virology·Y GhendonG Krivtsov
Jun 28, 2011·Nanomedicine : Nanotechnology, Biology, and Medicine·Yu-Ling LinKuang-Wen Liao
Jul 21, 2011·European Journal of Immunology·Nicholas W KinJohn F Kearney

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Citations

Nov 23, 2012·Journal of the Royal Society, Interface·Akhilesh Kumar Shakya, Kutty Selva Nandakumar
May 23, 2014·Pharmaceutical Research·Preety SahdevJames J Moon

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Methods Mentioned

BETA
fluorescence-activated cell sorting
FACS
ELISA
transfection

Software Mentioned

SAS

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