Liposome-encapsulated hemoglobin ameliorates tumor hypoxia and enhances radiation therapy to suppress tumor growth in mice

Artificial Organs
Chieko MurayamaMunetaka Haida

Abstract

We hypothesize that liposome-encapsulated hemoglobin with high O₂ affinity (P₅₀0₂ = 12 mm Hg, h-LEH) may increase O₂ delivery to hypoxic tumors and enhance radiation therapy synergistically to suppress tumor growth. First, h-LEH (5, 10, and 20 mL/kg) was intravenously infused 30 min before radiation (20 Gy) of SCCVII tumor grown in C3H/HeN mice. Second, 10 mL/kg of h-LEH was administered 30, 60, 90, and 120 min prior to radiation to determine optimal timing. Tumor size was monitored thereafter to titrate tumor growth suppression. Third, additional mice with SCCVII tumor were infused with h-LEH or empty liposome (EL), and tumors were excised at various time points for immunohistochemical examination of h-LEH and hypoxia-inducible factor-1α (HIF-1α). h-LEH was most effective at 10 mL/kg in comparison to 5 or 20 mL/kg of h-LEH or EL. Tumor growth was most suppressed when the interval between h-LEH infusion and radiation was shortest, 30 min. As a result, 10 mL/kg of h-LEH infusion 30 min prior to radiation prolonged 5-fold tumor-growth time from 20.0 days (radiation and EL) to 26.5 days, P<0.01, synergy ratio 1.42. While human hemoglobin (h-LEH) was detected in tumors 0.5 to 24 h after administration, HIF-1α accumulation was spars...Continue Reading

References

Apr 18, 1990·Journal of the National Cancer Institute·D J ChaplinM J Trotter
Jun 15, 1993·International Journal of Radiation Oncology, Biology, Physics·C MurayamaT Mori
Mar 1, 1996·International Journal of Radiation Oncology, Biology, Physics·R MurataM Abe
Apr 6, 2004·Methods in Enzymology·Peter VaupelMichael Höckel
Jul 16, 2005·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·S MasunagaK Ono
Sep 1, 2006·Strahlentherapie und Onkologie : Organ der Deutschen Röntgengesellschaft ... [et al]·Arnulf MayerPeter Vaupel
Oct 19, 2006·Pharmacology & Therapeutics·KangAe LeeJohn J LaPres
Apr 19, 2007·Cancer Metastasis Reviews·Peter Vaupel, Arnulf Mayer
Sep 11, 2007·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Jens Overgaard
Oct 16, 2007·Cancer Treatment Reviews·Minghua YuRuijuan Xiu
Apr 30, 2008·JAMA : the Journal of the American Medical Association·Charles NatansonSidney M Wolfe
Nov 17, 2009·The Journal of Pharmacology and Experimental Therapeutics·Akira T KawaguchiHideo Tsukada
Dec 17, 2010·Cancer Research·Shingo MatsumotoMurali C Krishna
Feb 22, 2012·Artificial Organs·Tsuyoshi FukuiRica Tanaka

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Citations

Feb 22, 2012·Artificial Organs·Akira T Kawaguchi
Feb 22, 2012·Artificial Organs·Tsuyoshi FukuiRica Tanaka
May 13, 2014·Genome Biology and Evolution·Michael J GaudryFederico G Hoffmann
Nov 3, 2015·Asian Pacific Journal of Tropical Medicine·Tian-Yan ZhangBing-Jie Huo
Mar 6, 2013·Artificial Organs·Paul S Malchesky
Aug 27, 2014·Artificial Organs·Akira T Kawaguchi
Jul 30, 2014·Artificial Organs·Chieko MurayamaYasukazu Nagato
Apr 8, 2014·The International Journal of Biochemistry & Cell Biology·Laura TerraneoMichele Samaja
Sep 21, 2016·Journal of Translational Medicine·Linfeng XuHongqian Guo
Mar 23, 2017·Artificial Organs·Tsuyoshi FukuiRica Tanaka
Apr 16, 2021·Advanced Science·Sheng PengFujun Zhang
Apr 12, 2019·Chemical Reviews·Bowen YangJianlin Shi
Jan 31, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Ruyuan SongShuhuai Yao

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