PMID: 6982072Sep 9, 1982Paper

Liposome-mediated delivery of ribosome inactivating proteins to cells in vitro

Biochimica Et Biophysica Acta
D P McIntosh, T Heath

Abstract

This study describes the liposome-mediated delivery of toxins to a variety of cells in vitro. Gelonin, a potent inhibitor of protein synthesis from Gelonium multiflorum, was delivered to the cytoplasm of TLX5 lymphoma cells most effectively by phosphatidylserine vesicles. These liposomes were also capable in inhibiting protein synthesis in XC (transformed rat fibroblasts) and phytohaemagglutinin-stimulated CBA mouse lymphocytes. Phosphatidylcholine liposomes had no capacity to deliver their contents to the cytoplasm, but the addition of cholesterol to the vesicle membrane resulted in an increased capacity. Delivery events were enhanced further by the addition of mixed bovine brain gangliosides to the membrane in the ratio 5:5:1 phosphatidylcholine/ cholesterol/gangliosides. The addition of cholesterol to phosphatidylserine vesicles failed to increase the inhibitory effects of the gelonin liposomes. The A chain of diphtheria toxin encapsulated in phosphatidylserine liposomes had no inhibitory effect on the level of protein synthesis in TLX5 or Daudi cells.

References

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Aug 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·M J ClemensI M London
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Feb 3, 1981·Biochemistry·J Van Renswoude, D Hoekstra
Sep 8, 2009·Applied and Environmental Microbiology·Lutz GeueFranz J Conraths

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Citations

Apr 27, 1999·Journal of Biomolecular Structure & Dynamics·R C MorrisM S Elliott
Dec 19, 2007·Journal of Biomedical Materials Research. Part a·Ping-Shan LaiPei-Jen Lou
Jun 1, 1984·Analytical Biochemistry·A J CutlerP D Shargool
Jan 20, 1986·FEBS Letters·F Stirpe, L Barbieri
Nov 20, 2018·Nanomedicine·Fatemeh FarjadianMichael R Hamblin
Jul 2, 2003·The Journal of Biological Chemistry·Chester J ProvodaKyung-Dall Lee
Dec 21, 1993·Biochimica Et Biophysica Acta·L BarbieriF Stirpe
Jan 16, 1986·Biochimica Et Biophysica Acta·E Yoshihara, T Nakae
Nov 23, 1983·Biochimica Et Biophysica Acta·V K Jansons, E A Panzner
Jun 3, 2021·International Journal of Molecular Sciences·Olga ShilovaSergey Deyev
Jul 21, 2006·The Journal of Physical Chemistry. B·Yun YanHonglan Fu

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